{"id":"W1989591859","doi":"10.1117/12.655618","title":"Permeabilization and cell surgery using femtosecond laser pulses: an emerging tool for cellular manipulation","year":2006,"lang":"en","type":"article","venue":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","topic":"Laser Material Processing Techniques","field":"Engineering","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cryoprotectant; Femtosecond; Biophysics; Cytoplasm; Intracellular; Decellularization; Extracellular; Cell biology; Cryopreservation; Laser; Cell; Materials science; Chemistry; Biology; Extracellular matrix; Optics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0005913383,0.0003279631,0.0003879403,0.000144683,0.0001112217,0.0002214076,0.0003547291,0.0002118696,0.000008188435],"category_scores_gemma":[0.000142302,0.0003149485,0.0002928409,0.0002191256,0.0001056663,0.001097171,0.0000685591,0.0001437542,2.404665e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000157701,"about_ca_system_score_gemma":0.00002075844,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001535884,"about_ca_topic_score_gemma":3.225574e-7,"domain_scores_codex":[0.9981945,2.50273e-8,0.0007277863,0.0003525306,0.0003597095,0.0003654255],"domain_scores_gemma":[0.9986593,0.0001077396,0.0002522808,0.00005950045,0.0008532373,0.00006799957],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004197544,0.00006445965,0.0007011401,0.001860453,0.0000735588,5.67163e-8,0.0001013611,0.003255539,0.9640546,0.02876307,0.0008896257,0.0001941506],"study_design_scores_gemma":[0.000400978,0.00007263417,0.0003836445,0.0001954704,0.0001085572,0.000005289011,0.0002223701,0.3162321,0.6787211,0.002749281,0.0005464243,0.0003622155],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968681,0.00009396217,0.001443748,0.0001204414,0.0002472862,0.0006229134,0.00005831692,0.0002911045,0.000254155],"genre_scores_gemma":[0.8935755,0.00003150003,0.1056067,0.00001977222,0.0004487894,0.0001185589,0.00005090936,0.0001099056,0.00003835358],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3129765,"threshold_uncertainty_score":0.9999303,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0154662877899558,"score_gpt":0.2257176559821753,"score_spread":0.2102513681922195,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}