{"id":"W1989591859","doi":"10.1117/12.655618","title":"Permeabilization and cell surgery using femtosecond laser pulses: an emerging tool for cellular manipulation","year":2006,"lang":"en","type":"article","venue":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","topic":"Laser Material Processing Techniques","field":"Engineering","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cryoprotectant; Femtosecond; Biophysics; Cytoplasm; Intracellular; Decellularization; Extracellular; Cell biology; Cryopreservation; Laser; Cell; Materials science; Chemistry; Biology; Extracellular matrix; Optics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002628268,0.0003232349,0.0002411714,0.0002451282,0.0002509654,0.0003665597,0.0002735072,0.0005659347,0.0007192444],"category_scores_gemma":[0.0001994733,0.0001425399,0.0001712439,0.0002061361,0.0006313677,0.0005693978,0.0004050118,0.0006591823,0.000286306],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004202486,"about_ca_system_score_gemma":0.0003072482,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004932814,"about_ca_topic_score_gemma":0.0007612478,"domain_scores_codex":[0.9998446,0.00001824775,0.000007544511,0.00003313307,0.00007503164,0.00002143909],"domain_scores_gemma":[0.9998642,0.00004574568,0.00003853915,0.00001981107,0.0000188985,0.00001277962],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001014194,0.000006358615,0.00007916728,0.00006220517,0.000002855004,0.00003190501,0.00002674199,0.00007607825,0.9922296,0.0005783549,0.0001092629,0.00678728],"study_design_scores_gemma":[0.00000481032,0.0001115475,0.00142942,0.00001451687,0.000007001619,0.0005809613,0.00004419601,0.001436114,0.9850678,0.0006106001,0.01067817,0.0000147406],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6324569,0.05487772,0.2999687,0.002887568,0.0004354514,0.0001623963,0.0003233483,0.001134956,0.007752845],"genre_scores_gemma":[0.8335669,0.03771969,0.1188012,0.001014612,0.0002097906,0.0002198235,0.0003354171,0.0001210858,0.008011564],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007192444,"threshold_uncertainty_score":0.003049076,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0154662877899558,"score_gpt":0.2257176559821753,"score_spread":0.2102513681922195,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}