{"id":"W1990261189","doi":"10.1139/w01-100","title":"Development of a method for mRNA differential display in filamentous fungi: comparison of mRNA differential display reverse transcription polymerase chain reaction and cDNA amplified fragment length polymorphism in <i>Leptosphaeria maculans</i>","year":2001,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Plant tissue culture and regeneration","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Plant Biotechnology Institute","funders":"","keywords":"Complementary DNA; Biology; Differential display; Molecular biology; Leptosphaeria maculans; Primer (cosmetics); Southern blot; Reverse transcriptase; Gene expression; Polymerase chain reaction; Rapid amplification of cDNA ends; Reverse transcription polymerase chain reaction; Gene; Northern blot; Amplified fragment length polymorphism; Genetics; Molecular cloning; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001947443,0.001188377,0.001162397,0.001808143,0.000550568,0.0008474406,0.00114262,0.0008711141,0.001142124],"category_scores_gemma":[0.002332993,0.0008571947,0.0008337756,0.0008700963,0.0007320093,0.0004583753,0.0008266076,0.002618986,0.001602217],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005298877,"about_ca_system_score_gemma":0.0005719764,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000561828,"about_ca_topic_score_gemma":0.001179707,"domain_scores_codex":[0.9983808,0.0003690887,0.0001510674,0.0004531913,0.0004702742,0.0001757189],"domain_scores_gemma":[0.9983759,0.0008007732,0.000170354,0.000226783,0.0003282251,0.00009793175],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004004724,0.00002666394,0.0001412116,0.0000672098,0.0000087296,0.00003576898,0.00002210514,0.00005982589,0.9947433,0.0001975888,0.00006198658,0.004595461],"study_design_scores_gemma":[0.00003392109,0.0002294075,0.001946252,0.00001824264,0.00003774825,0.0004655807,0.00002393428,0.003241781,0.9880216,0.0002468833,0.005710412,0.00002424478],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07840773,0.0009817842,0.9154812,0.0002519337,0.0002823287,0.0008584059,0.001098854,0.001184562,0.001453242],"genre_scores_gemma":[0.09524846,0.001411965,0.8934554,0.0002320198,0.00007147362,0.002625447,0.002765522,0.0005187018,0.00367104],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001947443,"threshold_uncertainty_score":0.01029921,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01510033679884985,"score_gpt":0.2547742635243184,"score_spread":0.2396739267254685,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}