{"id":"W1990298216","doi":"10.1186/1471-213x-11-8","title":"Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos","year":2011,"lang":"en","type":"article","venue":"BMC Developmental Biology","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada; Université Laval","keywords":"Polysome; Biology; Messenger RNA; Oocyte; RNA; Translation (biology); Homologous chromosome; Oogenesis; Gametogenesis; Cell biology; Protein biosynthesis; Embryo; P-bodies; Transcriptome; Cytoplasm; Nucleic acid; Molecular biology; Gene; Embryogenesis; Biochemistry; Ribosome; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002573857,0.0002193462,0.000225563,0.00008952889,0.0001317288,0.0000315881,0.0003142536,0.0002073824,0.0001477419],"category_scores_gemma":[0.00008848873,0.0001962016,0.00005746451,0.0000995341,0.0001087406,0.00001051558,0.0004735494,0.0001019564,0.0001116344],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002354021,"about_ca_system_score_gemma":0.0001494042,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002849435,"about_ca_topic_score_gemma":0.0006109542,"domain_scores_codex":[0.9983909,0.000167524,0.0002497257,0.0005890534,0.00008910948,0.0005137444],"domain_scores_gemma":[0.9993852,0.00004444981,0.00005108727,0.0001895098,0.00004619046,0.0002835811],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003322172,0.00001929952,0.2218542,0.000004883047,0.00005133953,0.000005423834,0.0002201135,7.666647e-8,0.770816,0.0001847674,0.00006606619,0.006445707],"study_design_scores_gemma":[0.0005029788,0.0004767135,0.3601907,0.00001024776,0.000007239543,0.00004843264,0.0003008056,0.000003255166,0.6332934,0.0004643324,0.004414645,0.0002872477],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9891671,0.0004224847,0.008724171,0.00005159091,0.00007295112,0.0002331645,0.00005080005,0.00001899968,0.001258718],"genre_scores_gemma":[0.8327384,0.00003902534,0.1654921,0.0003645509,0.000089667,0.00003415286,0.00009494895,0.0000218486,0.001125279],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1567679,"threshold_uncertainty_score":0.800087,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03698202056177334,"score_gpt":0.2964789490831226,"score_spread":0.2594969285213493,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}