{"id":"W1991569079","doi":"10.1074/jbc.m310460200","title":"Calpain Activation by Cooperative Ca2+ Binding at Two Non-EF-hand Sites","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Calpain Protease Function and Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"","keywords":"Cooperativity; Chemistry; Active site; Binding site; Mutant; Biophysics; Calpain; Stereochemistry; Salt bridge; Protease; Gene isoform; Cooperative binding; Binding domain; Enzyme; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002001471,0.0001470582,0.0001753208,0.00001837093,0.0001454462,0.00002840707,0.00009562513,0.0002385908,0.0001197743],"category_scores_gemma":[0.0002043636,0.0001006234,0.0001335075,0.00007993294,0.000127333,0.000007406483,0.00004520348,0.0001325849,0.000005943782],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001405879,"about_ca_system_score_gemma":0.00007916073,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004196961,"about_ca_topic_score_gemma":0.000001966465,"domain_scores_codex":[0.9992127,0.00003604257,0.0002891203,0.0001901065,0.0001155427,0.0001565313],"domain_scores_gemma":[0.9993545,0.00002189306,0.000248366,0.0001016719,0.000153695,0.0001198954],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000261043,0.00008691721,0.001035261,0.000007544849,0.0000416301,0.000004117976,0.00002117886,0.0003844241,0.9962342,0.000001830375,0.001578685,0.0003431987],"study_design_scores_gemma":[0.001377363,0.000399179,0.0003779711,0.00002302542,0.000009315485,0.0000867753,0.00006383938,0.0000177471,0.985269,0.0000299802,0.01220646,0.0001393667],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9956881,0.0002098377,0.003357964,0.000296062,0.00006396944,0.00007846684,0.00001532033,0.000005359807,0.0002849221],"genre_scores_gemma":[0.9977354,0.000107101,0.0002055262,0.0001515147,0.0004949548,0.000004042464,0.0002097276,0.000008380535,0.00108336],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01096519,"threshold_uncertainty_score":0.4103304,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01305391637541349,"score_gpt":0.252921940588795,"score_spread":0.2398680242133815,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}