{"id":"W1991569079","doi":"10.1074/jbc.m310460200","title":"Calpain Activation by Cooperative Ca2+ Binding at Two Non-EF-hand Sites","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Calpain Protease Function and Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"","keywords":"Cooperativity; Chemistry; Active site; Binding site; Mutant; Biophysics; Calpain; Stereochemistry; Salt bridge; Protease; Gene isoform; Cooperative binding; Binding domain; Enzyme; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001808487,0.0003524583,0.0002528871,0.0000933273,0.0001365128,0.0003905506,0.0003723866,0.0003321809,0.0007835213],"category_scores_gemma":[0.0002102739,0.000229975,0.0002159735,0.0001018505,0.0002121939,0.000197192,0.0003230054,0.0004245844,0.000279905],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000372633,"about_ca_system_score_gemma":0.0001534138,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003721033,"about_ca_topic_score_gemma":0.0006677061,"domain_scores_codex":[0.999809,0.0000179584,0.0000187571,0.00005889732,0.00005608077,0.00003933192],"domain_scores_gemma":[0.9998235,0.00002925238,0.00006553819,0.00002541731,0.00001514209,0.00004112885],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008415215,0.00001977268,0.0003251558,0.00001271648,0.000002766541,0.00005916124,0.000007043735,0.0000425199,0.9987019,0.0000440601,0.00001500971,0.0006857875],"study_design_scores_gemma":[0.00005176337,0.0001032023,0.008936482,0.000004098827,0.000008812567,0.0005144808,0.00001527339,0.002169902,0.98737,0.00007865889,0.0007411487,0.00000604168],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9978542,0.0003434102,0.001323478,0.00002979807,0.000008939174,0.000009273163,0.00002495727,0.0000419066,0.0003639709],"genre_scores_gemma":[0.9980605,0.00009671235,0.001151251,0.00001811187,0.000004928091,0.000007080625,0.00007215119,0.000006533948,0.0005826069],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007835213,"threshold_uncertainty_score":0.002703667,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01305391637541349,"score_gpt":0.252921940588795,"score_spread":0.2398680242133815,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}