{"id":"W1992116854","doi":"10.1016/j.ab.2011.04.037","title":"Stabilization of hypoxia-inducible factor-1α in buffer containing cobalt chloride for Western blot analysis","year":2011,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Cancer, Hypoxia, and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"National Institute of Biomedical Imaging and Bioengineering; National Institutes of Health; Canadian Institutes of Health Research; Alberta Innovates; Alberta Innovates - Health Solutions","keywords":"Western blot; Chemistry; Cobalt; Hypoxia (environmental); Buffer (optical fiber); Hypoxia-inducible factors; Protease; Biochemistry; Degradation (telecommunications); Enzyme; Molecular biology; Chromatography; Oxygen; Biology; Inorganic chemistry; Organic chemistry; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002093802,0.0002124428,0.0004382748,0.0001072133,0.00003277646,0.00001365449,0.0002270903,0.000276183,0.00008374375],"category_scores_gemma":[0.000258815,0.0002127859,0.0002954401,0.0004017494,0.0001216111,0.000007174194,0.00007904041,0.00009773274,0.000001853032],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003014992,"about_ca_system_score_gemma":0.0001391134,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000150884,"about_ca_topic_score_gemma":0.0001363317,"domain_scores_codex":[0.9984671,0.00002307113,0.0004635391,0.0005355133,0.0001644801,0.0003462611],"domain_scores_gemma":[0.9989908,0.00003274569,0.0001496599,0.0004910097,0.0001921445,0.0001436708],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006007283,0.0002540331,0.06017118,0.000111708,0.0006244211,0.000001174835,0.0001429502,0.00004032777,0.9369254,0.0001328251,0.0001802648,0.0008150339],"study_design_scores_gemma":[0.000900089,0.0001471085,0.01623623,0.00001605771,0.0004022122,0.00000186373,0.000158382,0.000325652,0.9791487,0.00005643741,0.00233948,0.0002677485],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9941403,0.0005901041,0.00386921,0.00002724294,0.0001322237,0.000174059,0.0001155445,0.00001087627,0.0009404432],"genre_scores_gemma":[0.9982521,0.0001279287,0.0003069484,0.00009833918,0.0003377698,0.00003026502,0.0002616782,0.0000217937,0.0005632004],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04393495,"threshold_uncertainty_score":0.8677157,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03628498556866845,"score_gpt":0.2860228391412943,"score_spread":0.2497378535726259,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}