{"id":"W1992310655","doi":"10.1371/journal.pone.0047259","title":"Boundaries of the Origin of Replication: Creation of a pET-28a-Derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors","year":2012,"lang":"en","type":"article","venue":"PLoS ONE","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; University of Toronto; Ontario Innovation Trust; California HIV/AIDS Research Program","keywords":"Replicon; Plasmid; PBR322; Biology; Origin of replication; Genetics; Cloning vector; Multiple cloning site; Vector (molecular biology); DNA replication; Molecular biology; DNA; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001015158,0.00009073098,0.0001977152,0.00001767377,0.00004412432,0.000006878503,0.0001114786,0.0000226735,0.000008743555],"category_scores_gemma":[0.00005463548,0.00006377781,0.00003313021,0.00009104652,0.0001911716,0.000005390332,0.00002064706,0.00004132279,2.670141e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008236454,"about_ca_system_score_gemma":0.00007076329,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005307479,"about_ca_topic_score_gemma":0.00003347417,"domain_scores_codex":[0.9993678,0.0000254445,0.0001749191,0.0001337829,0.0001803141,0.000117706],"domain_scores_gemma":[0.9992253,0.00001984036,0.000149721,0.0004075186,0.0001650204,0.00003260461],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001852285,0.0002093916,0.08919577,0.0001388342,0.0002034901,1.675278e-7,0.0002656817,0.0001810841,0.9094882,0.0001057698,0.00001198284,0.0000143961],"study_design_scores_gemma":[0.0006071678,0.0003611013,0.04971972,0.0001403138,0.0001337189,0.000005334216,0.00009761329,0.0001963103,0.9485053,0.000002155171,0.0001497528,0.00008152318],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9951007,0.0003446949,0.004003265,0.00005505646,0.00001856622,0.0002423375,0.00002197847,0.000005820283,0.0002076201],"genre_scores_gemma":[0.9963905,0.00001801363,0.003437456,0.00001353493,0.0000379955,0.00002059579,0.00002284595,0.00001268695,0.00004637864],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03947605,"threshold_uncertainty_score":0.2600784,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01753815977324634,"score_gpt":0.2625208597136745,"score_spread":0.2449826999404282,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}