{"id":"W1992310655","doi":"10.1371/journal.pone.0047259","title":"Boundaries of the Origin of Replication: Creation of a pET-28a-Derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors","year":2012,"lang":"en","type":"article","venue":"PLoS ONE","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; University of Toronto; Ontario Innovation Trust; California HIV/AIDS Research Program","keywords":"Replicon; Plasmid; PBR322; Biology; Origin of replication; Genetics; Cloning vector; Multiple cloning site; Vector (molecular biology); DNA replication; Molecular biology; DNA; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001014895,0.000653723,0.000788187,0.0005395514,0.0004755811,0.0009654863,0.001012747,0.001038368,0.002633828],"category_scores_gemma":[0.001125869,0.0007462412,0.0007044211,0.0006839373,0.0007260878,0.0006725772,0.0007825807,0.002057343,0.004498309],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006750504,"about_ca_system_score_gemma":0.0007038353,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005563768,"about_ca_topic_score_gemma":0.0006128208,"domain_scores_codex":[0.9989892,0.0001885424,0.0001694103,0.0002561602,0.0002816796,0.000115005],"domain_scores_gemma":[0.9991583,0.0001915129,0.0001973187,0.0001986949,0.0001150276,0.0001391184],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001149052,0.00006885839,0.000213818,0.0001178657,0.000006436934,0.0001232119,0.00007110615,0.0001839651,0.9933603,0.001222772,0.0002783337,0.004238468],"study_design_scores_gemma":[0.0000447252,0.0001888719,0.001246997,0.00003237358,0.00002369855,0.000559927,0.00004099826,0.002875018,0.9635738,0.0002780365,0.03111256,0.00002320275],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.652928,0.003248947,0.324723,0.0007911662,0.0005791744,0.001732974,0.005038358,0.002737944,0.008220459],"genre_scores_gemma":[0.6493582,0.00170075,0.3162801,0.0002844231,0.00007189555,0.001174379,0.01362499,0.001168033,0.01633722],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002633828,"threshold_uncertainty_score":0.008811057,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01753815977324634,"score_gpt":0.2625208597136745,"score_spread":0.2449826999404282,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}