{"id":"W1993442664","doi":"10.1006/prep.2001.1542","title":"Construction, Expression, and Characterization of a Baculovirally Expressed Catalytic Domain of Human Matrix Metalloproteinase-9","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Protease and Inhibitor Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":false,"ca_institutions":"Thunder Bay Regional Health Sciences Centre","funders":"","keywords":"Sf9; Matrix metalloproteinase; Recombinant DNA; Chemistry; Molecular biology; Substrate (aquarium); Sepharose; Biochemistry; Enzyme; Matrix (chemical analysis); Metalloproteinase; Gene; Biology; Chromatography","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000722785,0.0004268661,0.0004371129,0.0004512298,0.000572587,0.0008408917,0.0008960688,0.0005052716,0.0007662906],"category_scores_gemma":[0.0005976807,0.0004235647,0.0005146919,0.0005790093,0.0004098798,0.0004060465,0.0003932999,0.001435432,0.0009964306],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000902503,"about_ca_system_score_gemma":0.0005609153,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001969387,"about_ca_topic_score_gemma":0.001653419,"domain_scores_codex":[0.9996056,0.00006524847,0.00005499432,0.00006640898,0.0001391015,0.00006860778],"domain_scores_gemma":[0.9995851,0.0001163105,0.00006718105,0.00007436967,0.00007213582,0.00008495065],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008839281,0.00007548786,0.0001633841,0.00006506431,0.00001105335,0.0001306346,0.00006542687,0.0001441299,0.9969086,0.001141936,0.0001321279,0.001073726],"study_design_scores_gemma":[0.00005231702,0.0001009155,0.002562234,0.00001670839,0.00003434518,0.0004823291,0.00006341068,0.00237683,0.9784946,0.0003194678,0.01548233,0.00001455969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9263037,0.003484578,0.05901295,0.0008406517,0.0004085495,0.0005098028,0.005708445,0.0002830061,0.003448301],"genre_scores_gemma":[0.9138916,0.002472255,0.05671631,0.0002468194,0.00007736334,0.000296622,0.0164275,0.0001665184,0.009704963],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001969387,"threshold_uncertainty_score":0.006548166,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008095275143289744,"score_gpt":0.2365822508583979,"score_spread":0.2284869757151081,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}