{"id":"W1993660866","doi":"10.1021/bi400034m","title":"Strategies for Modulating the pH-Dependent Activity of a Family 11 Glycoside Hydrolase","year":2013,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Chemistry; Stereochemistry; Active site; Protonation; Nucleophile; Hydrolase; Population; Deprotonation; Substrate (aquarium); Enzyme; Biochemistry; Catalysis; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002564704,0.0005071181,0.0002157876,0.0001305128,0.00009159518,0.0003783526,0.000252911,0.0003194694,0.0005191487],"category_scores_gemma":[0.0002137245,0.0001583066,0.0001883781,0.0001116307,0.0002367212,0.0002380734,0.0002831503,0.000746329,0.000192791],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002321707,"about_ca_system_score_gemma":0.0002090172,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003265531,"about_ca_topic_score_gemma":0.0005621451,"domain_scores_codex":[0.99984,0.00003073284,0.00002225294,0.00003207533,0.000047751,0.00002707636],"domain_scores_gemma":[0.9998516,0.00002761556,0.00006229855,0.00001487139,0.00001515325,0.00002845093],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000349837,0.00003355841,0.00006966168,0.00002332213,0.000004176215,0.00002219529,0.000006142597,0.0001220724,0.9984937,0.00007701248,0.00001273944,0.001100463],"study_design_scores_gemma":[0.00001698402,0.0003195684,0.0006090255,0.000003722706,0.00001181345,0.0001303942,0.00001328674,0.001065745,0.9963188,0.00005014202,0.001454397,0.000006120088],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9870141,0.001119235,0.009795762,0.0001834249,0.00004458895,0.00009515784,0.0001316987,0.0001100189,0.001506039],"genre_scores_gemma":[0.9827544,0.00126696,0.01469613,0.00009039803,0.00001076275,0.0000508697,0.0001569588,0.00003241252,0.0009411359],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005191487,"threshold_uncertainty_score":0.00173676,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0138025556104562,"score_gpt":0.2471821365859652,"score_spread":0.233379580975509,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}