{"id":"W1994587188","doi":"10.1016/s1385-299x(03)00020-5","title":"Organotypic slices in vitro: repeated, same-cell, high-resolution tracking of nuclear and cytoplasmic fluorescent signals in live, transfected cerebellar neurons by confocal microscopy","year":2003,"lang":"en","type":"article","venue":"Brain Research Protocols","topic":"Neuroscience and Neuropharmacology Research","field":"Neuroscience","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canada Research Chairs; University of Toronto","funders":"","keywords":"Cytoplasm; Confocal; Confocal microscopy; Fluorescence microscope; Fluorescence; In vitro; Transfection; Cell biology; Biology; Biophysics; Chemistry; Cell culture; Physics; Optics; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009960158,0.0009535541,0.001179805,0.0004199935,0.000920693,0.001145515,0.002026445,0.001268136,0.001105737],"category_scores_gemma":[0.0007227664,0.0008506852,0.001054427,0.0004358088,0.0008183846,0.001020932,0.001254108,0.003410574,0.001258793],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001411721,"about_ca_system_score_gemma":0.001044784,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01158926,"about_ca_topic_score_gemma":0.03319321,"domain_scores_codex":[0.9990302,0.00009785155,0.00009003476,0.0002866526,0.0002633525,0.000231889],"domain_scores_gemma":[0.9990231,0.0001859048,0.0001720434,0.0002775852,0.0002023184,0.000139011],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000460195,0.00002291166,0.00007258066,0.00003209954,0.00001144855,0.00006709458,0.0000807354,0.00009858456,0.998646,0.0001679981,0.00007250648,0.0006820403],"study_design_scores_gemma":[0.00003543652,0.0001744296,0.002204141,0.0000158165,0.00005019633,0.0003656207,0.0000641978,0.001661527,0.9934629,0.0001856679,0.001755391,0.00002465996],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7123969,0.00279068,0.2711425,0.0003997821,0.0004665905,0.0006997696,0.002146947,0.001969886,0.007986939],"genre_scores_gemma":[0.733033,0.002701211,0.246912,0.0003517771,0.00008752479,0.001166761,0.00264162,0.001364415,0.01174174],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.01158926,"threshold_uncertainty_score":0.02304363,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07393849723431732,"score_gpt":0.3953591283172513,"score_spread":0.321420631082934,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}