{"id":"W1996738992","doi":"10.1111/1574-6941.12219","title":"Commercial DNA extraction kits impact observed microbial community composition in permafrost samples","year":2013,"lang":"en","type":"article","venue":"FEMS Microbiology Ecology","topic":"Climate change and permafrost","field":"Earth and Planetary Sciences","cited_by":115,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Oak Ridge National Laboratory; Biological and Environmental Research; Office of Science; UT-Battelle; Battelle; U.S. Department of Energy","keywords":"Biology; Permafrost; DNA extraction; DNA; Microbial population biology; Extraction (chemistry); Composition (language); Ecology; Bacteria; Polymerase chain reaction; Genetics; Chromatography; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000630497,0.0006591031,0.0004018636,0.0009942311,0.0004528688,0.0005605406,0.0003877738,0.0005112084,0.00146398],"category_scores_gemma":[0.001053134,0.0002498931,0.0002343949,0.0008850102,0.0003751996,0.0002805406,0.0003592038,0.0002959165,0.0007683816],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002939499,"about_ca_system_score_gemma":0.0004827669,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001640641,"about_ca_topic_score_gemma":0.004969625,"domain_scores_codex":[0.998801,0.0001713632,0.0001110482,0.0003827699,0.0004221675,0.0001115866],"domain_scores_gemma":[0.9994563,0.0001517082,0.0001406692,0.00002912475,0.0001833893,0.00003874308],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001679932,0.00006438691,0.0157732,0.0002249155,0.00001822939,0.00005742069,0.0001961426,0.00009446959,0.9673757,0.00005115662,0.00009194318,0.01588434],"study_design_scores_gemma":[0.00003081087,0.0008583026,0.2669453,0.0001426152,0.00008774371,0.0005027382,0.0005977113,0.001497238,0.7152193,0.0002535488,0.01381575,0.00004908134],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9805796,0.001049684,0.01184077,0.00008526199,0.00002939071,0.0002910313,0.003524771,0.0002115701,0.002387976],"genre_scores_gemma":[0.8699892,0.001925175,0.09666508,0.000473895,0.00004513835,0.001248466,0.01983122,0.0001555416,0.009666256],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001640641,"threshold_uncertainty_score":0.004897475,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06821271917867236,"score_gpt":0.2725307058642816,"score_spread":0.2043179866856092,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}