{"id":"W1996863846","doi":"10.1155/2013/754319","title":"High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in<i>Escherichia coli</i>","year":2013,"lang":"en","type":"article","venue":"BioMed Research International","topic":"Antimicrobial Peptides and Activities","field":"Immunology and Microbiology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke; Biotechnology Research Institute","funders":"National High-tech Research and Development Program; Foundation for the Author of National Excellent Doctoral Dissertation of the People's Republic of China","keywords":"Intein; Escherichia coli; Antimicrobial; Pichia pastoris; Peptide; Fusion protein; Expression vector; Cleavage (geology); Biochemistry; Biology; Antimicrobial peptides; Recombinant DNA; Chemistry; Microbiology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006057813,0.0001037617,0.0001491745,0.0001633011,0.0002921532,0.00006652666,0.0004934854,0.0001333699,0.0001968819],"category_scores_gemma":[0.0001410117,0.00006222597,0.00005405286,0.0002211751,0.0008054272,0.0001863551,0.0003865504,0.0003825781,0.00003350248],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001051792,"about_ca_system_score_gemma":0.00008573323,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004691385,"about_ca_topic_score_gemma":0.00008130243,"domain_scores_codex":[0.9987788,0.0002973775,0.0002950806,0.0002143493,0.0001220605,0.0002923778],"domain_scores_gemma":[0.9991378,0.0002485237,0.0001323599,0.0002307605,0.0002338813,0.00001665222],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005396688,0.00006044706,0.0009681501,0.00003064989,0.00003507136,4.620102e-7,0.0003127641,0.00001563884,0.976632,0.001102441,0.02058751,0.0002008711],"study_design_scores_gemma":[0.0005333039,0.00003445914,0.007375595,0.0002789718,0.000005300259,0.0000178668,0.002295351,0.0002749793,0.9825461,0.0001496702,0.006391616,0.00009680872],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9963075,0.0001168936,0.0001687931,0.001611543,0.0009937711,0.0004531916,0.00004046136,0.00001375658,0.0002940686],"genre_scores_gemma":[0.9980915,0.00003237992,0.0002433275,0.00006303602,0.00009238735,0.00003174471,0.00003009541,0.00001122121,0.001404289],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01419589,"threshold_uncertainty_score":0.7092002,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04102753769885585,"score_gpt":0.292226973630126,"score_spread":0.2511994359312701,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}