{"id":"W1996863846","doi":"10.1155/2013/754319","title":"High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in<i>Escherichia coli</i>","year":2013,"lang":"en","type":"article","venue":"BioMed Research International","topic":"Antimicrobial Peptides and Activities","field":"Immunology and Microbiology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke; Biotechnology Research Institute","funders":"National High-tech Research and Development Program; Foundation for the Author of National Excellent Doctoral Dissertation of the People's Republic of China","keywords":"Intein; Escherichia coli; Antimicrobial; Pichia pastoris; Peptide; Fusion protein; Expression vector; Cleavage (geology); Biochemistry; Biology; Antimicrobial peptides; Recombinant DNA; Chemistry; Microbiology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001505914,0.0005326206,0.0003104802,0.0001454334,0.0001523514,0.000419355,0.0001956139,0.0002701627,0.0003912268],"category_scores_gemma":[0.0001110586,0.0001355177,0.0002707544,0.0003574619,0.0001610111,0.0002479545,0.0004010533,0.0005211135,0.0003784194],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002372887,"about_ca_system_score_gemma":0.0001887355,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005165393,"about_ca_topic_score_gemma":0.0007100002,"domain_scores_codex":[0.9998726,0.00001461311,0.00001325196,0.00002939577,0.0000404153,0.00002977631],"domain_scores_gemma":[0.9999547,0.000006057756,0.00001179008,0.000006484214,0.00001069787,0.00001028552],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001701031,0.000008398729,0.0001053159,0.00004258951,0.00000290089,0.0000518725,0.000008901741,0.00007018596,0.9989356,0.00003836897,0.00002923421,0.0006895594],"study_design_scores_gemma":[0.000004551071,0.00004363649,0.001429748,0.000007468123,0.00000928089,0.000218529,0.0000221805,0.000782512,0.9946225,0.00003242102,0.002822411,0.000004824083],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9673408,0.002230507,0.02692307,0.0001735611,0.00005207652,0.00006980688,0.00102113,0.0002178955,0.001971044],"genre_scores_gemma":[0.9669989,0.002036654,0.02217495,0.00009057692,0.00001747548,0.00005071073,0.003910569,0.0001108503,0.00460926],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005326206,"threshold_uncertainty_score":0.001721621,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04102753769885585,"score_gpt":0.292226973630126,"score_spread":0.2511994359312701,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}