{"id":"W1997763166","doi":"10.1016/j.jbiotec.2008.04.006","title":"Expression of SEAP (secreted alkaline phosphatase) by baculovirus mediated transduction of HEK 293 cells in a hollow fiber bioreactor system","year":2008,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":false,"ca_institutions":"Sanofi (Canada); McGill University; Precision BioLogic (Canada); BioPhage Pharma (Canada)","funders":"","keywords":"Multiplicity of infection; Chinese hamster ovary cell; HEK 293 cells; Molecular biology; Cell culture; Trichostatin A; Biology; Recombinant DNA; Alkaline phosphatase; Reporter gene; Cell biology; Chemistry; Gene expression; Histone deacetylase; Biochemistry; Gene; Histone; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001645063,0.0001710882,0.000420448,0.0002496739,0.00003580047,0.000002779596,0.0002313995,0.000574243,0.00006628488],"category_scores_gemma":[0.00006949939,0.0001407689,0.0002012906,0.0002800226,0.0002009576,0.00001178718,0.00005021559,0.000225312,0.000003107546],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002885559,"about_ca_system_score_gemma":0.0001151584,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006779836,"about_ca_topic_score_gemma":0.000007524719,"domain_scores_codex":[0.9985499,0.00009451638,0.0007485858,0.0002101972,0.0001993197,0.0001975047],"domain_scores_gemma":[0.9987618,0.00002060028,0.0007250213,0.0002703204,0.0001375631,0.00008463809],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006465935,0.0002518652,0.0003253988,0.0000832175,0.00005466066,0.00002899206,0.00003707199,0.00007474915,0.9903078,0.000005027106,0.007860583,0.0003240177],"study_design_scores_gemma":[0.001744738,0.001048705,0.0002190521,0.0001537059,0.00002808042,0.000147351,0.0001243102,0.00002348725,0.9895842,0.000007100033,0.006791443,0.0001278065],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952139,0.003516601,0.0004709573,0.00009241761,0.0002825098,0.000136816,0.0002473664,0.00001224062,0.00002720002],"genre_scores_gemma":[0.9974052,0.001925575,0.0004310718,0.00002585971,0.00006685902,0.000004269601,0.00008190897,0.00001890035,0.00004039856],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002191267,"threshold_uncertainty_score":0.5740388,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006587931586415486,"score_gpt":0.219473236731775,"score_spread":0.2128853051453595,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}