{"id":"W1997763166","doi":"10.1016/j.jbiotec.2008.04.006","title":"Expression of SEAP (secreted alkaline phosphatase) by baculovirus mediated transduction of HEK 293 cells in a hollow fiber bioreactor system","year":2008,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":false,"ca_institutions":"Sanofi (Canada); McGill University; Precision BioLogic (Canada); BioPhage Pharma (Canada)","funders":"","keywords":"Multiplicity of infection; Chinese hamster ovary cell; HEK 293 cells; Molecular biology; Cell culture; Trichostatin A; Biology; Recombinant DNA; Alkaline phosphatase; Reporter gene; Cell biology; Chemistry; Gene expression; Histone deacetylase; Biochemistry; Gene; Histone; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005635292,0.0003709076,0.0006332624,0.0002998598,0.0004052064,0.0007066015,0.0005315788,0.0004075246,0.0009150447],"category_scores_gemma":[0.0002362326,0.0003289005,0.0005487995,0.000363124,0.0004031175,0.0005055983,0.0004489335,0.001255307,0.0009049774],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006422027,"about_ca_system_score_gemma":0.0005117042,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001132522,"about_ca_topic_score_gemma":0.001170808,"domain_scores_codex":[0.9995471,0.00005755136,0.00006066716,0.00009046323,0.0001773928,0.0000668022],"domain_scores_gemma":[0.9998186,0.00004747745,0.00002962041,0.00003015474,0.00004035446,0.00003372767],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004632113,0.00001958467,0.00006578772,0.00002562081,0.000004883739,0.00003034632,0.00002351487,0.00007053705,0.9988445,0.0002850317,0.00004118516,0.0005426822],"study_design_scores_gemma":[0.00001138471,0.00005814875,0.000550818,0.000003447222,0.0000128084,0.00007950584,0.00001453755,0.00174629,0.9954386,0.00005418209,0.002024918,0.00000532976],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9049728,0.0008520258,0.08605365,0.0004701951,0.000271935,0.0003980209,0.002433985,0.0007946026,0.003752768],"genre_scores_gemma":[0.8984557,0.0009722087,0.07693408,0.0001841192,0.00009409342,0.0003500924,0.006261548,0.0002248474,0.0165233],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001132522,"threshold_uncertainty_score":0.004659474,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006587931586415486,"score_gpt":0.219473236731775,"score_spread":0.2128853051453595,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}