{"id":"W1998520008","doi":"10.1139/w00-043","title":"Purification and characterization of a common soil component which inhibits the polymerase chain reaction","year":2000,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":149,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"","keywords":"Polymerase chain reaction; Chemistry; Microorganism; DNA; Chromatography; Biochemistry; Solubility; DNA extraction; Polymerase; Molecular biology; Biology; Bacteria; Organic chemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001974794,0.00007544478,0.0001178491,0.00006039623,0.00008741061,0.00000676325,0.0001251439,0.0001443951,0.00002490801],"category_scores_gemma":[0.00001198842,0.00005941575,0.00003807871,0.00007515404,0.000145455,0.000003125389,0.000008464617,0.0001000535,0.000001362334],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001286704,"about_ca_system_score_gemma":0.0001557926,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004833437,"about_ca_topic_score_gemma":0.003281315,"domain_scores_codex":[0.9993958,0.0001045376,0.0002571553,0.0001079322,0.00001599933,0.0001185064],"domain_scores_gemma":[0.9994532,0.000006191073,0.0001956431,0.0001588744,0.0001041606,0.00008187179],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002203974,0.00001414096,0.0005218638,0.000002938369,0.00001981094,7.655598e-7,0.00003922671,0.000002084195,0.9935659,0.0001123494,0.0002207213,0.00547814],"study_design_scores_gemma":[0.0002291811,0.0002157594,0.01106902,0.00001656561,0.00002141062,0.0005664416,0.00002048646,0.00000933334,0.9161499,0.00003820172,0.07157926,0.00008447376],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9971619,0.0004804729,0.0002803169,0.0017537,0.0000401947,0.0001168413,0.00005749394,0.000001625482,0.0001075084],"genre_scores_gemma":[0.9987097,0.000393487,0.00007751428,0.0003687926,0.00006714141,0.000005418839,0.0002886619,0.000007236202,0.00008209109],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07741605,"threshold_uncertainty_score":0.2422904,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005004736521749193,"score_gpt":0.2036709093839786,"score_spread":0.1986661728622294,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}