{"id":"W1998528347","doi":"10.1074/jbc.m210919200","title":"A Fast-acting, Modular-structured Staphylokinase Fusion with Kringle-1 from Human Plasminogen as the Fibrin-targeting Domain Offers Improved Clot Lysis Efficacy","year":2003,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"National Heart, Lung, and Blood Institute; University of Notre Dame; Heart and Stroke Foundation of Canada","keywords":"Staphylokinase; Pichia pastoris; Fusion protein; Lysis; Fibrin; Kringle domain; Chemistry; Plasmin; Biochemistry; Biology; Recombinant DNA; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001385148,0.0003711379,0.0002323184,0.0001357782,0.00006312497,0.0002428024,0.0002067617,0.0003093572,0.000478451],"category_scores_gemma":[0.0001384887,0.0001073436,0.0002595071,0.0001458588,0.0001313931,0.0002445845,0.0001923821,0.000457926,0.0002672159],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002854975,"about_ca_system_score_gemma":0.000129376,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002436339,"about_ca_topic_score_gemma":0.0001978364,"domain_scores_codex":[0.999898,0.00001460399,0.00001125805,0.0000218546,0.00003376208,0.00002058539],"domain_scores_gemma":[0.999884,0.00001603928,0.00005254981,0.00000880773,0.00001010483,0.00002847889],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004585694,0.000008904538,0.00006302768,0.00001620784,0.00000209365,0.00002893397,0.000004392204,0.00009488395,0.998634,0.00004411079,0.00001450447,0.001042969],"study_design_scores_gemma":[0.00001949721,0.0002369328,0.001138162,0.000002782626,0.00001045968,0.000364022,0.000005356403,0.001238181,0.9955361,0.00002304493,0.001419811,0.00000577406],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9865575,0.001536949,0.01099727,0.00008519227,0.00001741228,0.00002664842,0.0001704745,0.0001106407,0.0004979224],"genre_scores_gemma":[0.9885917,0.0005012976,0.009371413,0.00002677117,0.000006476176,0.00001199739,0.0003382518,0.0000156884,0.00113628],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000478451,"threshold_uncertainty_score":0.002071381,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01023499924096138,"score_gpt":0.2289150428373168,"score_spread":0.2186800435963554,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}