{"id":"W1999063456","doi":"10.1021/bi015538b","title":"Independent Lec1A CHO Glycosylation Mutants Arise from Point Mutations in <i>N</i>-Acetylglucosaminyltransferase I That Reduce Affinity for Both Substrates. Molecular Consequences Based on the Crystal Structure of GlcNAc-TI<sup>,</sup>","year":2001,"lang":"en","type":"article","venue":"Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Cancer Institute; U.S. Public Health Service","keywords":"Asparagine; Mutant; Glycosylation; Amino acid; Glycan; Biochemistry; Mutagenesis; Chemistry; N-linked glycosylation; Arginine; Point mutation; Chinese hamster ovary cell; Mutation; Site-directed mutagenesis; Stereochemistry; Glycoprotein; Gene; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001232764,0.001234173,0.0003676761,0.0006197926,0.0003561984,0.0005021627,0.0006445877,0.0005938768,0.001826974],"category_scores_gemma":[0.0002990997,0.0002836648,0.0007517679,0.0005934183,0.0006804101,0.0001993041,0.0007865956,0.001043142,0.001020517],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009931098,"about_ca_system_score_gemma":0.0003608564,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003252076,"about_ca_topic_score_gemma":0.003198721,"domain_scores_codex":[0.9996734,0.00003087218,0.00004792579,0.00006798091,0.0001227452,0.00005714334],"domain_scores_gemma":[0.9995053,0.00006763654,0.0002101403,0.00005082675,0.00004907297,0.000117019],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001684603,0.00004474272,0.000479947,0.00002731718,0.00002234276,0.0004188226,0.00003522963,0.0001175262,0.9971458,0.0001574149,0.00008412037,0.001298169],"study_design_scores_gemma":[0.00006528735,0.000297815,0.01227536,0.00001062591,0.00006922476,0.002103186,0.00008921909,0.001311446,0.9800074,0.00005917997,0.003676596,0.00003467596],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9895099,0.000485988,0.003989296,0.0001304689,0.00008367209,0.0001568593,0.002396154,0.0002064454,0.003041173],"genre_scores_gemma":[0.9808124,0.0006121477,0.006638774,0.0001459645,0.00002388883,0.0001828785,0.004581951,0.00028949,0.006712473],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003252076,"threshold_uncertainty_score":0.007205486,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01958044261602365,"score_gpt":0.2683726346799556,"score_spread":0.248792192063932,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}