{"id":"W1999063456","doi":"10.1021/bi015538b","title":"Independent Lec1A CHO Glycosylation Mutants Arise from Point Mutations in <i>N</i>-Acetylglucosaminyltransferase I That Reduce Affinity for Both Substrates. Molecular Consequences Based on the Crystal Structure of GlcNAc-TI<sup>,</sup>","year":2001,"lang":"en","type":"article","venue":"Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Cancer Institute; U.S. Public Health Service","keywords":"Asparagine; Mutant; Glycosylation; Amino acid; Glycan; Biochemistry; Mutagenesis; Chemistry; N-linked glycosylation; Arginine; Point mutation; Chinese hamster ovary cell; Mutation; Site-directed mutagenesis; Stereochemistry; Glycoprotein; Gene; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002644192,0.0003027446,0.0002421938,0.00007956238,0.0001326149,0.0000633427,0.0003796313,0.0003657113,0.0002673838],"category_scores_gemma":[0.0005259773,0.0002605207,0.0001629488,0.0002601676,0.0002803338,0.00001307228,0.00004110602,0.0002757785,0.000001896333],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006963988,"about_ca_system_score_gemma":0.0005941027,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002380447,"about_ca_topic_score_gemma":0.0001668229,"domain_scores_codex":[0.9980237,0.0001314517,0.0004070476,0.0006001136,0.0004487125,0.0003889535],"domain_scores_gemma":[0.9987663,0.0001469388,0.0001532914,0.0006022454,0.0001698581,0.0001613334],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000759021,0.0001680304,0.002216037,0.00004902204,0.00004218934,0.00001695858,0.00008250112,0.005171792,0.9911007,0.00002369814,0.00009331964,0.0002766752],"study_design_scores_gemma":[0.001761327,0.0001558177,0.001478012,0.00006829744,0.00002150701,0.00001215631,0.0004459264,0.003866424,0.9913703,0.0003149617,0.0002311339,0.0002741714],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946421,0.0002532554,0.002091981,0.0006998097,0.00003785436,0.0009979677,0.0009670598,0.00001618984,0.0002937909],"genre_scores_gemma":[0.9972593,0.00005809345,0.0005604077,0.0002369363,0.00008126497,0.0001242223,0.001594672,0.00003245485,0.00005266563],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002617191,"threshold_uncertainty_score":0.9999847,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01958044261602365,"score_gpt":0.2683726346799556,"score_spread":0.248792192063932,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}