{"id":"W1999971214","doi":"10.1039/b915986j","title":"An integrated global strategy for cell lysis, fractionation, enrichment and mass spectrometric analysis of phosphorylated peptides","year":2010,"lang":"en","type":"article","venue":"Molecular BioSystems","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Phosphoproteomics; Phosphopeptide; Fractionation; Phosphorylation; Chemistry; Phosphatase; Lysis; Fragmentation (computing); Chromatography; Protein phosphorylation; Biochemistry; Computational biology; Biology; Protein kinase A","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001396955,0.0001484704,0.0002594964,0.0001875022,0.00007307337,0.00004471536,0.0001644498,0.0001546917,0.00006844832],"category_scores_gemma":[0.00002197126,0.0001452497,0.0001183564,0.001290442,0.00003415839,0.00005492076,0.00001511812,0.0001141664,7.932703e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006880033,"about_ca_system_score_gemma":0.00003413939,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000170533,"about_ca_topic_score_gemma":0.00002670213,"domain_scores_codex":[0.9990098,0.00001178003,0.0003301847,0.0003312555,0.0001524938,0.0001644505],"domain_scores_gemma":[0.999005,0.00002620294,0.0002559486,0.0003871673,0.0002446272,0.00008101856],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001242388,0.00009754424,0.002925242,0.00004337013,0.0002249905,7.151235e-7,0.000007624606,0.0003214221,0.9937698,0.001994438,0.00001489695,0.0005875353],"study_design_scores_gemma":[0.0002356105,0.00004994014,0.0004065988,0.000005945512,0.0003688839,0.000001896884,0.0001185573,0.01723291,0.9793887,0.000962042,0.001061687,0.0001672468],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7454089,0.0001983322,0.2531572,0.0000182733,0.00001559061,0.0002329955,0.0003025242,0.0000703943,0.000595793],"genre_scores_gemma":[0.9672616,0.0000268864,0.03199283,0.000008333353,0.00001666369,0.0001373548,0.000492021,0.0000140553,0.00005028761],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2218527,"threshold_uncertainty_score":0.5923112,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006126744908198627,"score_gpt":0.2738786485547607,"score_spread":0.2677519036465621,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}