{"id":"W1999971214","doi":"10.1039/b915986j","title":"An integrated global strategy for cell lysis, fractionation, enrichment and mass spectrometric analysis of phosphorylated peptides","year":2010,"lang":"en","type":"article","venue":"Molecular BioSystems","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Phosphoproteomics; Phosphopeptide; Fractionation; Phosphorylation; Chemistry; Phosphatase; Lysis; Fragmentation (computing); Chromatography; Protein phosphorylation; Biochemistry; Computational biology; Biology; Protein kinase A","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008718914,0.001531279,0.002078633,0.001015932,0.0004248079,0.0009524634,0.001051647,0.0007640509,0.0007970174],"category_scores_gemma":[0.0003363555,0.0005553269,0.0007881802,0.0007406955,0.0004742671,0.0009282712,0.001162536,0.002177798,0.001152704],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004168144,"about_ca_system_score_gemma":0.0009677717,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003617225,"about_ca_topic_score_gemma":0.001039248,"domain_scores_codex":[0.9994672,0.00005494823,0.00005254159,0.0002049213,0.0001604775,0.00005995438],"domain_scores_gemma":[0.9998084,0.00002773641,0.00002597251,0.00005323941,0.00004907096,0.00003562863],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000245881,0.00001894676,0.00005968676,0.00007717709,0.00001217759,0.00001408377,0.000006767754,0.00007412639,0.9926686,0.0001261497,0.0001312088,0.006786405],"study_design_scores_gemma":[0.00001589791,0.0001734213,0.001331422,0.00001241834,0.00006269068,0.0003671782,0.0000139728,0.00163894,0.9872267,0.0002557147,0.008882226,0.0000193361],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07125827,0.005503834,0.9155946,0.0003781144,0.000327728,0.0008616365,0.00210331,0.002151159,0.001821376],"genre_scores_gemma":[0.1185737,0.006760122,0.8620229,0.0004643893,0.0001188379,0.001448192,0.005238407,0.0003409624,0.005032428],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002078633,"threshold_uncertainty_score":0.004611075,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006126744908198627,"score_gpt":0.2738786485547607,"score_spread":0.2677519036465621,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}