{"id":"W2000087003","doi":"10.1074/jbc.m111.295857","title":"In Vitro Reconstruction of the Chain Termination Reaction in Biosynthesis of the Escherichia coli O9a O-Polysaccharide","year":2011,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"Alberta Innovates; University of Alberta; University of Guelph","funders":"Canadian Institutes of Health Research; Alberta Innovates","keywords":"Chemistry; Methylation; Biochemistry; Periplasmic space; Escherichia coli; Biosynthesis; Phosphorylation; Acceptor; ATP-binding cassette transporter; Enzyme; Stereochemistry; Transporter; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003668021,0.0004739377,0.0003367153,0.0001220708,0.0001714888,0.0005065278,0.0004209841,0.0004458939,0.0005315778],"category_scores_gemma":[0.0003183699,0.0002356695,0.0003664964,0.0002403574,0.0001982893,0.0002045855,0.0002862615,0.0008675577,0.0005975586],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006173207,"about_ca_system_score_gemma":0.0003937694,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001868842,"about_ca_topic_score_gemma":0.001815539,"domain_scores_codex":[0.9997131,0.0001002633,0.00002821321,0.00004401235,0.0000694995,0.00004486797],"domain_scores_gemma":[0.999818,0.00005739686,0.00004494043,0.00002942073,0.00002333779,0.00002692755],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001568728,0.0000512783,0.0001411958,0.00003905503,0.000007977585,0.00006268026,0.00003002591,0.001445953,0.9967148,0.0003634343,0.00002727739,0.0009594571],"study_design_scores_gemma":[0.00001294469,0.0001862215,0.0004246474,0.000007861892,0.00001111548,0.00004907206,0.00001586553,0.00478598,0.9920368,0.00007490542,0.002386823,0.000007715817],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.97981,0.001078299,0.01586662,0.0001288576,0.00005555319,0.00007470183,0.000595597,0.0001081394,0.002282182],"genre_scores_gemma":[0.974591,0.001005466,0.01904723,0.00003370085,0.00001021537,0.00005592389,0.001410001,0.00007269143,0.003773758],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001868842,"threshold_uncertainty_score":0.004479051,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01496803767180137,"score_gpt":0.2133207027729151,"score_spread":0.1983526651011137,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}