{"id":"W2000265342","doi":"10.1101/pdb.prot080242","title":"Immunostaining for Markers of Apoptosis in the <i>Caenorhabditis elegans</i> Germline","year":2014,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children","funders":"Canadian Institutes of Health Research","keywords":"Caenorhabditis elegans; Germline; Immunostaining; Biology; Model organism; Cell biology; Somatic cell; Apoptosis; Mutant; Immunofluorescence; Antibody; Molecular biology; Genetics; Immunohistochemistry; Gene; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002215623,0.0003451339,0.0001625278,0.0007561662,0.0006513725,0.0002964897,0.0004136289,0.0003385604,0.002163711],"category_scores_gemma":[0.000131347,0.000281703,0.0003006146,0.0002615447,0.0002846734,0.0002924371,0.0002717235,0.0008848773,0.0008271785],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000436708,"about_ca_system_score_gemma":0.0004213827,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001729279,"about_ca_topic_score_gemma":0.005900409,"domain_scores_codex":[0.9998226,0.00001101002,0.00002073851,0.0000458577,0.00006505341,0.00003481411],"domain_scores_gemma":[0.9998156,0.00002599468,0.00004250403,0.00002377095,0.00005104917,0.00004110425],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00001023072,0.000007150057,0.0001543592,0.00003511242,0.000002387999,0.0000377193,0.00001369947,0.00002473192,0.9983711,0.0002095488,0.0001520261,0.0009820967],"study_design_scores_gemma":[0.00001890874,0.0001366281,0.0138035,0.00005844267,0.00002534099,0.0007206555,0.00005801287,0.0008884572,0.9725155,0.0001716851,0.01159078,0.0000121367],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.8886546,0.004612132,0.07685006,0.0005997021,0.0002743583,0.0004404733,0.003447403,0.001903564,0.02321765],"genre_scores_gemma":[0.7987503,0.009613326,0.1515696,0.0006126061,0.00009093367,0.000869642,0.006488713,0.0005611969,0.03144372],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.002163711,"threshold_uncertainty_score":0.007238328,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01109192968917501,"score_gpt":0.2547063739356347,"score_spread":0.2436144442464597,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}