{"id":"W2000987352","doi":"10.1089/dna.2009.0915","title":"Efficient Transfection of Endothelial Cells by a Double-Pulse Electroporation Method","year":2009,"lang":"en","type":"article","venue":"DNA and Cell Biology","topic":"Microbial Inactivation Methods","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre hospitalier de l'Université Laval","funders":"","keywords":"Electroporation; Transfection; Biology; Umbilical vein; Endothelial stem cell; Cell culture; Cell biology; Lipofectamine; Molecular biology; Human umbilical vein endothelial cell; Gene; In vitro; Genetics; Vector (molecular biology); Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001031477,0.0006171733,0.0008479047,0.0006625402,0.0003366507,0.0007104428,0.0007462309,0.0006637287,0.001914246],"category_scores_gemma":[0.0004883521,0.0003366463,0.0004842426,0.0005302103,0.0004646598,0.0003621682,0.0005181343,0.001598025,0.001336523],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002462587,"about_ca_system_score_gemma":0.0002963832,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002433839,"about_ca_topic_score_gemma":0.0004412165,"domain_scores_codex":[0.9990503,0.0001670731,0.0001344415,0.0002305283,0.000311812,0.0001058479],"domain_scores_gemma":[0.9996146,0.0001285907,0.00005505694,0.0000993781,0.00006312563,0.0000392459],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000330641,0.00002460483,0.00003054443,0.00005160667,0.000005474525,0.0000290753,0.00001737741,0.00004261664,0.9968787,0.000190857,0.0001201963,0.002575823],"study_design_scores_gemma":[0.0000128343,0.00008840379,0.0003005091,0.000004714228,0.00001164489,0.0002058689,0.000004737877,0.0008691084,0.9937982,0.00005639478,0.004639942,0.000007714677],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2400883,0.002563403,0.7474395,0.0002664255,0.0004721668,0.001266464,0.001576164,0.00175615,0.004571446],"genre_scores_gemma":[0.4640951,0.004053435,0.5126606,0.0002589411,0.0001178527,0.001766206,0.00419607,0.0005161457,0.01233566],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001914246,"threshold_uncertainty_score":0.006403744,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006691990324794487,"score_gpt":0.2739840380975827,"score_spread":0.2672920477727882,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}