{"id":"W2001050841","doi":"10.1002/bip.22149","title":"Design, expression, and purification of de novo transmembrane “hairpin” peptides","year":2012,"lang":"en","type":"article","venue":"Biopolymers","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Circular dichroism; Chemistry; Folding (DSP implementation); Transmembrane protein; Sodium dodecyl sulfate; Gel electrophoresis; Transmembrane domain; Biochemistry; Membrane protein; Biophysics; Polyacrylamide gel electrophoresis; Peptide; Membrane; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001157912,0.00009862814,0.00009357796,0.00002980338,0.00004013532,0.000005331762,0.0000772846,0.000115075,0.00002438619],"category_scores_gemma":[0.00001338671,0.00008318476,0.00003929641,0.00004311324,0.00009997586,0.000006716765,0.00001611903,0.0000348798,0.000001260194],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003776064,"about_ca_system_score_gemma":0.00003173036,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001065285,"about_ca_topic_score_gemma":8.683976e-7,"domain_scores_codex":[0.9994295,0.00004016164,0.0001267462,0.0001389418,0.00006894327,0.0001957639],"domain_scores_gemma":[0.9996502,0.000007148998,0.00005171231,0.0001715638,0.00002219387,0.00009711468],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005871042,0.00003413683,0.002999936,0.00002617708,0.00001057886,2.082995e-7,0.0001930262,0.000001217067,0.9939263,0.00001917721,0.00008747713,0.002643029],"study_design_scores_gemma":[0.0003020826,0.00006634554,0.004153403,0.000009398645,0.00002827541,0.00002353688,0.0001250901,0.00000111686,0.9932413,0.000002858516,0.001941948,0.0001047122],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9954058,0.001446245,0.002647185,0.00005444983,0.0001123339,0.0001161084,0.000008307556,0.00000842127,0.0002011399],"genre_scores_gemma":[0.996892,0.0001833914,0.002420464,0.00006736808,0.0001801876,0.00001164569,0.00002654786,0.00001296257,0.0002054862],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002538317,"threshold_uncertainty_score":0.3392176,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0146577579124696,"score_gpt":0.251199478868507,"score_spread":0.2365417209560374,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}