{"id":"W2001050841","doi":"10.1002/bip.22149","title":"Design, expression, and purification of de novo transmembrane “hairpin” peptides","year":2012,"lang":"en","type":"article","venue":"Biopolymers","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Circular dichroism; Chemistry; Folding (DSP implementation); Transmembrane protein; Sodium dodecyl sulfate; Gel electrophoresis; Transmembrane domain; Biochemistry; Membrane protein; Biophysics; Polyacrylamide gel electrophoresis; Peptide; Membrane; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004976663,0.000330856,0.0002968869,0.0002309476,0.0002021589,0.0004630566,0.0004002105,0.0003781439,0.0005736292],"category_scores_gemma":[0.0005010426,0.000295064,0.0003140275,0.0002493878,0.0001529292,0.0003351641,0.0003506624,0.0008232594,0.0005022692],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003127073,"about_ca_system_score_gemma":0.0002802096,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001897342,"about_ca_topic_score_gemma":0.0002739924,"domain_scores_codex":[0.9997067,0.0000681478,0.00004459449,0.00006147042,0.00007695882,0.00004206696],"domain_scores_gemma":[0.9997664,0.00004738188,0.00006213832,0.0000254519,0.00005458423,0.00004412949],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002636256,0.00003785479,0.00009920957,0.0000326879,0.000004311765,0.00005883553,0.00002839007,0.0004956221,0.9968588,0.0003787071,0.00004279045,0.001936391],"study_design_scores_gemma":[0.00002157431,0.0003176623,0.0006225253,0.000007493558,0.00001522311,0.0001745384,0.00002793383,0.002853473,0.9892117,0.0001454947,0.006593498,0.000009001415],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8969901,0.001405732,0.09715896,0.0002484816,0.0001274734,0.0006543167,0.0008456969,0.0003252405,0.002244015],"genre_scores_gemma":[0.8644195,0.002062079,0.1263666,0.0001615741,0.0000506445,0.0003146852,0.002544453,0.000125218,0.003955347],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005736292,"threshold_uncertainty_score":0.002631962,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0146577579124696,"score_gpt":0.251199478868507,"score_spread":0.2365417209560374,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}