{"id":"W2001695232","doi":"10.1016/j.funbio.2013.04.001","title":"Optimization of a real-time PCR assay for the detection of the quarantine pathogen Melampsora medusae f. sp. deltoidae","year":2013,"lang":"en","type":"article","venue":"Fungal Biology","topic":"Bioenergy crop production and management","field":"Agricultural and Biological Sciences","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"Natural Resources Canada","funders":"","keywords":"Biology; Phytosanitary certification; Mycosphaerella; Internal transcribed spacer; Quarantine; Botany; Pathogen; Basidiomycota; PEST analysis; Polymerase chain reaction; Urediniospore; Ribosomal RNA; Horticulture; Microbiology; Ecology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00239951,0.001578411,0.001309938,0.0009315418,0.0004673343,0.001058631,0.001172074,0.0009978185,0.001658271],"category_scores_gemma":[0.002069718,0.001327394,0.001075599,0.0005430293,0.0009074074,0.000666405,0.0006375129,0.001542456,0.001145827],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005911452,"about_ca_system_score_gemma":0.000780276,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007249802,"about_ca_topic_score_gemma":0.002533766,"domain_scores_codex":[0.9963992,0.0007671725,0.0003459693,0.001040445,0.001169675,0.0002775215],"domain_scores_gemma":[0.9984504,0.0007618455,0.0002145992,0.000134782,0.0003503101,0.00008811191],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008735753,0.00009748263,0.0005229299,0.00005957913,0.000007872643,0.0000235164,0.0000531426,0.0001912389,0.9966227,0.00005817113,0.00003640083,0.002239681],"study_design_scores_gemma":[0.00004430348,0.001024302,0.005222232,0.00003467409,0.00007375907,0.0002251427,0.00009291257,0.006471551,0.9841774,0.0001122405,0.002481882,0.0000395245],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7172491,0.002062838,0.2714463,0.0004971435,0.0004304746,0.002241623,0.001568357,0.001191318,0.003312898],"genre_scores_gemma":[0.5760061,0.001055924,0.4109459,0.0003878941,0.00007910986,0.002978701,0.002999399,0.0001876641,0.005359343],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00239951,"threshold_uncertainty_score":0.01268995,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01266350775083891,"score_gpt":0.2037872556711277,"score_spread":0.1911237479202888,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}