{"id":"W2001756723","doi":"10.1074/jbc.m112.402057","title":"Automating Single Subunit Counting of Membrane Proteins in Mammalian Cells","year":2012,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":92,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université de Montréal","funders":"Canadian Institutes of Health Research","keywords":"Green fluorescent protein; Endoplasmic reticulum; Fluorescence recovery after photobleaching; Protein subunit; Xenopus; Photobleaching; Cytosol; Biology; Membrane protein; Cell biology; Biophysics; Biochemistry; Membrane; Fluorescence; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005145662,0.0001078872,0.0002132422,0.00002139181,0.00001972938,0.00000613448,0.0001435486,0.0002299154,0.00009493426],"category_scores_gemma":[0.00008587878,0.00008443593,0.0001287683,0.00007724822,0.00005570793,0.000007191717,0.00002744768,0.0001678035,8.916364e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001735907,"about_ca_system_score_gemma":0.00002901035,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004603524,"about_ca_topic_score_gemma":0.000001364707,"domain_scores_codex":[0.9990574,0.00003184949,0.0004752549,0.00009625055,0.0001231579,0.0002161569],"domain_scores_gemma":[0.9993626,0.00001411025,0.0003559913,0.0001016056,0.00008265556,0.00008300928],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007185991,0.0001805691,0.04142842,0.00007762729,0.00001360539,0.00000610006,0.0000366693,0.00001402071,0.9578692,0.000001739094,0.000004532223,0.0002956031],"study_design_scores_gemma":[0.0003528362,0.0001368305,0.008493994,0.00005141286,0.000009688546,0.00004688662,0.00009610247,0.000008270144,0.9888927,0.000006700724,0.001804695,0.00009991341],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979723,0.0007666711,0.0005341161,0.00002309468,0.00006047229,0.00005414058,0.00000465553,0.000003222026,0.0005813283],"genre_scores_gemma":[0.9985077,0.00006058002,0.0009565424,0.00001948472,0.0003095639,0.000001290014,0.00008572449,0.000007559214,0.00005149515],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03293442,"threshold_uncertainty_score":0.3443197,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01948585215583068,"score_gpt":0.2271877580224168,"score_spread":0.2077019058665861,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}