{"id":"W2001756723","doi":"10.1074/jbc.m112.402057","title":"Automating Single Subunit Counting of Membrane Proteins in Mammalian Cells","year":2012,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":92,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université de Montréal","funders":"Canadian Institutes of Health Research","keywords":"Green fluorescent protein; Endoplasmic reticulum; Fluorescence recovery after photobleaching; Protein subunit; Xenopus; Photobleaching; Cytosol; Biology; Membrane protein; Cell biology; Biophysics; Biochemistry; Membrane; Fluorescence; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001047246,0.0008510914,0.001324149,0.001361527,0.000506669,0.0009300417,0.001508502,0.000776838,0.001655823],"category_scores_gemma":[0.001238111,0.0006110898,0.000609246,0.00068633,0.0004597396,0.0006096936,0.0008213323,0.001077536,0.002788462],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005001914,"about_ca_system_score_gemma":0.0004528858,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005046928,"about_ca_topic_score_gemma":0.0009416277,"domain_scores_codex":[0.9985508,0.000138567,0.0001623214,0.0004593075,0.0006086193,0.00008024528],"domain_scores_gemma":[0.9989341,0.0003660388,0.0001510281,0.0002821932,0.0002087695,0.0000579276],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002937827,0.0000131323,0.0001983104,0.0000540107,0.000008491619,0.00002910851,0.00002776271,0.0001687529,0.9903017,0.0002659014,0.0001498241,0.008753577],"study_design_scores_gemma":[0.00001021212,0.00005344963,0.001561312,0.00001217205,0.00001905144,0.0003166259,0.00001606846,0.01052659,0.9823883,0.0003509943,0.004722006,0.00002318054],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1189592,0.0008387144,0.8712726,0.0001399975,0.00008675869,0.0003324673,0.001327962,0.00514808,0.001894191],"genre_scores_gemma":[0.1320072,0.001212833,0.8601969,0.00009583851,0.00004906983,0.001042309,0.002147672,0.0007650909,0.002483027],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001655823,"threshold_uncertainty_score":0.005539238,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01948585215583068,"score_gpt":0.2271877580224168,"score_spread":0.2077019058665861,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}