{"id":"W2001793440","doi":"10.1046/j.1365-2958.2000.02189.x","title":"Topological analysis and role of the transmembrane domain in polar targeting of PilS, a <i>Pseudomonas aeruginosa</i> sensor kinase","year":2000,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary General Hospital; University of Calgary","funders":"Medical Research Council Canada; University of British Columbia; University of Calgary","keywords":"Transmembrane protein; Biology; Transmembrane domain; Cell biology; Cytoplasm; Pilin; Topology (electrical circuits); Membrane topology; Pilus; Membrane protein; Biophysics; Biochemistry; Membrane; Gene; Escherichia coli; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009356407,0.0001782088,0.00008001731,0.0002859813,0.0002237901,0.0003180853,0.0001139618,0.0001654498,0.0003240476],"category_scores_gemma":[0.0002467398,0.00008675151,0.0001251467,0.0001435357,0.0003097925,0.0004032078,0.0001900176,0.0001864559,0.0002128689],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002597215,"about_ca_system_score_gemma":0.0001413732,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002521057,"about_ca_topic_score_gemma":0.0001968627,"domain_scores_codex":[0.999946,0.00001461308,0.000003875425,0.000008694704,0.00001367851,0.00001302484],"domain_scores_gemma":[0.9998671,0.00002182812,0.00004306029,0.00001058392,0.00001777501,0.00003968205],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000252294,0.00001533829,0.002251377,0.00006880509,0.000004701651,0.0001999452,0.00003797929,0.0008091397,0.9920585,0.001737016,0.00005041882,0.002514539],"study_design_scores_gemma":[0.00005344516,0.000497426,0.03901298,0.00003978964,0.00006194381,0.002437709,0.0004928327,0.02335362,0.9264207,0.002273775,0.005319554,0.00003626247],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970847,0.0003281863,0.001926442,0.00006821036,0.00000508046,0.000003118376,0.00005858617,0.00001845274,0.000507278],"genre_scores_gemma":[0.9984477,0.0002363396,0.0009090565,0.00001036817,0.000003757848,0.000002357983,0.0002136008,0.000004969783,0.0001718368],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0003240476,"threshold_uncertainty_score":0.00188446,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002018395207997274,"score_gpt":0.1807124509488783,"score_spread":0.1786940557408811,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}