{"id":"W2001793440","doi":"10.1046/j.1365-2958.2000.02189.x","title":"Topological analysis and role of the transmembrane domain in polar targeting of PilS, a <i>Pseudomonas aeruginosa</i> sensor kinase","year":2000,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary General Hospital; University of Calgary","funders":"Medical Research Council Canada; University of British Columbia; University of Calgary","keywords":"Transmembrane protein; Biology; Transmembrane domain; Cell biology; Cytoplasm; Pilin; Topology (electrical circuits); Membrane topology; Pilus; Membrane protein; Biophysics; Biochemistry; Membrane; Gene; Escherichia coli; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00019085,0.0001783759,0.0003875062,0.00009738686,0.0000332944,0.000004205461,0.000271665,0.0004453486,0.00006294302],"category_scores_gemma":[0.00002326882,0.0001350981,0.0002012548,0.0003591535,0.0004758234,0.000001299578,0.0001120657,0.0001422264,8.548823e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005775794,"about_ca_system_score_gemma":0.00002759049,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001303716,"about_ca_topic_score_gemma":0.0001100145,"domain_scores_codex":[0.9986547,0.0002433993,0.0003878772,0.0003899225,0.00003899451,0.0002851062],"domain_scores_gemma":[0.9994475,0.00001157256,0.0001105303,0.0003588111,0.00003650565,0.00003503738],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001287414,0.00008762861,0.005196572,0.00001275359,0.0001511399,0.00000607086,0.00004766709,0.00005071793,0.9930911,0.0001356411,0.000005641036,0.001086348],"study_design_scores_gemma":[0.0005589399,0.0002314495,0.002947332,0.000006597649,0.00008872855,0.00003639839,0.00006109703,0.000007640428,0.9889786,0.0001515257,0.006783808,0.0001478624],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966067,0.002531414,0.0001118725,0.0003142834,0.00002925998,0.000184454,0.0001035771,0.000004843762,0.0001135754],"genre_scores_gemma":[0.997758,0.0003495976,0.001553478,0.0001905081,0.00001682266,0.000005663901,0.00009033689,0.00001147542,0.00002408438],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006778167,"threshold_uncertainty_score":0.5509141,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002018395207997274,"score_gpt":0.1807124509488783,"score_spread":0.1786940557408811,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}