{"id":"W2002182475","doi":"10.1016/j.ab.2014.06.006","title":"Continuous colorimetric screening assay for detection of d-amino acid aminotransferase mutants displaying altered substrate specificity","year":2014,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Foundation for Innovation; University of Ottawa","keywords":"Substrate (aquarium); Colorimetry; Chemistry; Substrate specificity; Mutant; Biochemistry; Chromatography; Molecular biology; Enzyme; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004477725,0.0002628144,0.0003910804,0.00006120934,0.00009918895,0.00002895697,0.0002362767,0.0003219821,0.00001640915],"category_scores_gemma":[0.0005422218,0.000256636,0.0002726321,0.0002260432,0.0001640168,0.000006742819,0.00005622949,0.0001469955,0.000002878093],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001564077,"about_ca_system_score_gemma":0.00003846165,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001758279,"about_ca_topic_score_gemma":0.000005083624,"domain_scores_codex":[0.9983291,0.00005078375,0.0004509366,0.0005454423,0.0002024272,0.0004212775],"domain_scores_gemma":[0.9989785,0.00007676006,0.0001469777,0.0004156339,0.0001903861,0.0001917445],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002310389,0.00008328674,0.001413913,0.00007814139,0.00009840213,0.000001199533,0.000006069482,0.00001403714,0.9871582,0.00008428145,0.0001125366,0.01071884],"study_design_scores_gemma":[0.0009940689,0.0002264236,0.003541275,0.00001945088,0.0001379501,0.00001211121,0.00005055288,0.002380375,0.9897113,0.00002578597,0.002609543,0.0002911582],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8393775,0.0003571273,0.1591957,0.00002396238,0.0001291821,0.0001653954,0.00008805702,0.0000163411,0.0006467849],"genre_scores_gemma":[0.9974152,0.00004883308,0.001234127,0.00007618878,0.0006053573,0.00001834325,0.0001927402,0.00003028151,0.0003789597],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1580377,"threshold_uncertainty_score":0.9999886,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01368506031416063,"score_gpt":0.2525086187251365,"score_spread":0.2388235584109759,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}