{"id":"W2002909960","doi":"10.1107/s2053230x1402010x","title":"<i>In situ</i>proteolysis, crystallization and preliminary X-ray diffraction analysis of a V<sub>H</sub>H that binds listeria internalin B","year":2014,"lang":"en","type":"article","venue":"Acta Crystallographica Section F Structural Biology Communications","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; National Research Council Canada","funders":"National Research Council Canada; Western Economic Diversification Canada; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Program for Education and Research in Biotechnology, California State University; University of Saskatchewan; Canadian Light Source","keywords":"Proteolysis; Crystallization; Monoclinic crystal system; Trypsin; Listeria monocytogenes; Crystallography; Molecular biology; Listeria; Pathogen; Chemistry; Biology; Bacteria; Microbiology; Biochemistry; Crystal structure; Enzyme; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000313962,0.0004065031,0.000439883,0.0001407146,0.0002577952,0.0002543389,0.0004740694,0.0002480992,0.001245624],"category_scores_gemma":[0.0003169303,0.0001529685,0.0003182756,0.0004837305,0.000219859,0.0002080137,0.0001487088,0.0006162722,0.0005026617],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004088364,"about_ca_system_score_gemma":0.0003339349,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001743915,"about_ca_topic_score_gemma":0.001562006,"domain_scores_codex":[0.9998183,0.00002881662,0.00002477361,0.00003973567,0.00003988286,0.00004851256],"domain_scores_gemma":[0.9998772,0.00003178868,0.000023772,0.00001853874,0.00002975467,0.0000189618],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003529387,0.00007682147,0.0007538445,0.0001849128,0.0000219951,0.0004904626,0.0001129162,0.000503353,0.9908888,0.0005845493,0.001674134,0.004355175],"study_design_scores_gemma":[0.00004576729,0.0002300838,0.006919625,0.00001907319,0.00002519914,0.001013334,0.0001012958,0.001791471,0.9679242,0.0001735449,0.02173436,0.00002212478],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.970786,0.003070214,0.01473765,0.000618691,0.0001701869,0.000124009,0.00627887,0.0002198494,0.003994464],"genre_scores_gemma":[0.9199587,0.005981029,0.03711361,0.0003117651,0.00006882611,0.0001693549,0.02927059,0.0001856215,0.00694053],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001743915,"threshold_uncertainty_score":0.00416702,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0214411997992422,"score_gpt":0.3036775135668431,"score_spread":0.2822363137676009,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}