{"id":"W2002909960","doi":"10.1107/s2053230x1402010x","title":"<i>In situ</i>proteolysis, crystallization and preliminary X-ray diffraction analysis of a V<sub>H</sub>H that binds listeria internalin B","year":2014,"lang":"en","type":"article","venue":"Acta Crystallographica Section F Structural Biology Communications","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; National Research Council Canada","funders":"National Research Council Canada; Western Economic Diversification Canada; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Program for Education and Research in Biotechnology, California State University; University of Saskatchewan; Canadian Light Source","keywords":"Proteolysis; Crystallization; Monoclinic crystal system; Trypsin; Listeria monocytogenes; Crystallography; Molecular biology; Listeria; Pathogen; Chemistry; Biology; Bacteria; Microbiology; Biochemistry; Crystal structure; Enzyme; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003667155,0.0002239632,0.000558659,0.001376866,0.000240344,0.00002823144,0.0003207553,0.0002711213,0.00004147416],"category_scores_gemma":[0.0001276552,0.0001884719,0.0002456726,0.001607634,0.0006691705,0.0002142399,0.0003068333,0.0004901724,2.823541e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004661842,"about_ca_system_score_gemma":0.0000282381,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005192799,"about_ca_topic_score_gemma":0.001314874,"domain_scores_codex":[0.9981222,0.0005106481,0.0005420828,0.0003788734,0.0001835153,0.0002626437],"domain_scores_gemma":[0.9981168,0.0004105878,0.0003230174,0.0008283536,0.0001982033,0.0001230489],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"observational","study_design_scores_codex":[0.0005026461,0.00007995743,0.1277436,0.00004919412,0.0006349187,8.656378e-7,0.00018643,0.00001872778,0.8686998,0.0004280787,0.00002408126,0.001631672],"study_design_scores_gemma":[0.0007393056,0.0007051955,0.981161,0.00005744025,0.0006807439,0.00005122064,0.0001406407,0.007337369,0.003922509,0.0008978735,0.004095435,0.0002112449],"study_design_candidate":"observational","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9949702,0.000379872,0.0005897692,0.003166405,0.0001028103,0.0003851485,0.00004040079,0.00004338752,0.0003220064],"genre_scores_gemma":[0.9956682,0.002298382,0.0007854564,0.0002183992,0.00005444737,0.00005725994,0.0008698036,0.00001540693,0.00003269479],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.8647773,"threshold_uncertainty_score":0.768566,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0214411997992422,"score_gpt":0.3036775135668431,"score_spread":0.2822363137676009,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}