{"id":"W2003462220","doi":"10.1021/ac1005553","title":"Assay Development for the Determination of Phosphorylation Stoichiometry Using Multiple Reaction Monitoring Methods with and without Phosphatase Treatment: Application to Breast Cancer Signaling Pathways","year":2010,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":69,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; University of Victoria","funders":"National Institutes of Health; Canadian Institutes of Health Research; Genome British Columbia; National Cancer Institute; Genome Canada","keywords":"Phosphopeptide; Chemistry; Phosphorylation; Protein phosphorylation; Phosphatase; Dephosphorylation; Chromatography; Peptide; Phosphoproteomics; Biochemistry; Protein kinase A","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.008671259,0.002260579,0.001696491,0.002239456,0.0006849617,0.001528478,0.002143967,0.001769423,0.001207208],"category_scores_gemma":[0.005717633,0.001585883,0.001257642,0.001548053,0.001006424,0.001378478,0.001267191,0.002697624,0.001631602],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006687425,"about_ca_system_score_gemma":0.0009569146,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004721495,"about_ca_topic_score_gemma":0.0007899875,"domain_scores_codex":[0.9889538,0.002614712,0.0008667049,0.002312345,0.004969057,0.0002833077],"domain_scores_gemma":[0.9969439,0.001118058,0.000449211,0.0004822953,0.0008740005,0.0001325602],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006020792,0.00007617471,0.0005391486,0.0002865923,0.00005648388,0.00004794374,0.00007079857,0.0004485242,0.9787187,0.00042394,0.0002056443,0.01906589],"study_design_scores_gemma":[0.00001876214,0.0002582952,0.001449744,0.00002623335,0.00007040545,0.0003580402,0.00002267664,0.008782321,0.984309,0.0002495183,0.004413181,0.00004179066],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.04811592,0.002939311,0.9440866,0.000209556,0.0002508909,0.000710125,0.000301446,0.001873691,0.001512571],"genre_scores_gemma":[0.07378207,0.002844854,0.9181505,0.0002120275,0.00009096665,0.0017268,0.0005387502,0.0002544742,0.002399547],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.008671259,"threshold_uncertainty_score":0.0458585,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02980367961073893,"score_gpt":0.3513332192237796,"score_spread":0.3215295396130406,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}