{"id":"W2004369722","doi":"10.1128/aac.45.4.1168-1173.2001","title":"DNA Transformation of <i>Leishmania infantum</i> Axenic Amastigotes and Their Use in Drug Screening","year":2001,"lang":"en","type":"article","venue":"Antimicrobial Agents and Chemotherapy","topic":"Research on Leishmaniasis Studies","field":"Medicine","cited_by":106,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre hospitalier de l'Université Laval","funders":"Medical Research Council; Canadian Institutes of Health Research; Wellcome Trust; Washington University in St. Louis","keywords":"Amastigote; Leishmania infantum; Luciferase; Axenic; Miltefosine; Leishmania; Biology; Pharmacology; Electroporation; Leishmania major; Molecular biology; Leishmaniasis; Microbiology; Cell culture; Biochemistry; Transfection; Immunology; Visceral leishmaniasis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001801276,0.0001672577,0.0003557085,0.0001483887,0.00007342294,0.00004079599,0.00005651147,0.00005612259,0.00003422827],"category_scores_gemma":[0.00001718384,0.0001288148,0.00005572824,0.000195821,0.0001519391,0.0002750101,0.00002873243,0.0001349358,0.000001092955],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001330756,"about_ca_system_score_gemma":0.00001729102,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005073701,"about_ca_topic_score_gemma":0.0002438293,"domain_scores_codex":[0.9990928,0.00003292357,0.0002932351,0.0002094254,0.0001215568,0.0002500312],"domain_scores_gemma":[0.9996179,0.00005762019,0.00006740289,0.0001257308,0.00006088629,0.00007050625],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"observational","study_design_gemma":"observational","study_design_scores_codex":[0.001147907,0.000382389,0.781071,0.0003178154,0.0002531968,0.00002620089,0.01188115,0.000004677247,0.1349164,0.00006176823,0.007520115,0.06241738],"study_design_scores_gemma":[0.006235498,0.0001907961,0.9018519,0.0005061752,0.0000252597,0.00009880283,0.0008303797,0.0005165843,0.06573377,0.00004097147,0.02372813,0.0002417702],"study_design_candidate":"observational","study_design_consensus":"observational","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9956329,0.0001087536,0.0001687001,0.003350918,0.00001981405,0.0003643268,0.00001187972,0.0000208582,0.0003217938],"genre_scores_gemma":[0.987455,0.009746715,0.000346445,0.002215486,0.00003529121,0.000004083955,0.00001401204,0.00002075649,0.0001621914],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1207808,"threshold_uncertainty_score":0.5252917,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02512996241780061,"score_gpt":0.2679352163421248,"score_spread":0.2428052539243242,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}