{"id":"W2004369722","doi":"10.1128/aac.45.4.1168-1173.2001","title":"DNA Transformation of <i>Leishmania infantum</i> Axenic Amastigotes and Their Use in Drug Screening","year":2001,"lang":"en","type":"article","venue":"Antimicrobial Agents and Chemotherapy","topic":"Research on Leishmaniasis Studies","field":"Medicine","cited_by":106,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre hospitalier de l'Université Laval","funders":"Medical Research Council; Canadian Institutes of Health Research; Wellcome Trust; Washington University in St. Louis","keywords":"Amastigote; Leishmania infantum; Luciferase; Axenic; Miltefosine; Leishmania; Biology; Pharmacology; Electroporation; Leishmania major; Molecular biology; Leishmaniasis; Microbiology; Cell culture; Biochemistry; Transfection; Immunology; Visceral leishmaniasis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005564192,0.0009576858,0.0005572963,0.000598816,0.0002721635,0.0004679204,0.0005812747,0.0005136249,0.001664512],"category_scores_gemma":[0.0005179523,0.0003615028,0.0005351349,0.0008401109,0.0004552098,0.0002712205,0.0003780019,0.0009514345,0.001278029],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003746926,"about_ca_system_score_gemma":0.0003086351,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007806397,"about_ca_topic_score_gemma":0.001066696,"domain_scores_codex":[0.9994318,0.0001914743,0.00006358695,0.0001326604,0.0001225555,0.00005781817],"domain_scores_gemma":[0.9997017,0.00009840279,0.0000930399,0.00004142017,0.00005050909,0.00001484392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003260384,0.00001957292,0.00007292582,0.0001146853,0.000005437162,0.00002987218,0.00001973745,0.00006426941,0.9958051,0.0002081398,0.00008033539,0.003547185],"study_design_scores_gemma":[0.00002066971,0.0003087517,0.0007559984,0.0000129875,0.00003124422,0.0004304605,0.00001171538,0.0004507121,0.9856569,0.00009357926,0.01221624,0.0000107784],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5450517,0.01443528,0.4130892,0.0007772557,0.0004112088,0.003325561,0.007679151,0.001967909,0.01326281],"genre_scores_gemma":[0.6289499,0.01772547,0.3096024,0.0002846082,0.0001242594,0.002178563,0.02331328,0.0005735056,0.01724814],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001664512,"threshold_uncertainty_score":0.005568385,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02512996241780061,"score_gpt":0.2679352163421248,"score_spread":0.2428052539243242,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}