{"id":"W2004949070","doi":"10.3389/fbioe.2014.00067","title":"The Induction of Recombinant Protein Bodies in Different Subcellular Compartments Reveals a Cryptic Plastid-Targeting Signal in the 27-kDa γ-Zein Sequence","year":2014,"lang":"en","type":"article","venue":"Frontiers in Bioengineering and Biotechnology","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"Regione Lombardia; Austrian Science Fund","keywords":"Plastid; Endoplasmic reticulum; ER retention; Recombinant DNA; Fusion protein; Biogenesis; Signal peptide; Cell biology; Biology; Protein targeting; Organelle; Subcellular localization; Transit Peptide; Protein Sorting Signals; Protein subcellular localization prediction; Biochemistry; Membrane protein; Chloroplast; Cytoplasm; Gene; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003002311,0.000120488,0.0001681855,0.0001020822,0.00004648905,0.00000970087,0.0002018086,0.0001817612,2.72331e-7],"category_scores_gemma":[0.0000394633,0.00007623269,0.00002335795,0.0001237243,0.0001414308,0.000003128061,0.00003759047,0.0002038711,2.050672e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001700172,"about_ca_system_score_gemma":0.000007976665,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003027044,"about_ca_topic_score_gemma":0.00003979031,"domain_scores_codex":[0.9992026,0.00005693434,0.0002539926,0.0002081942,0.00005738957,0.0002208993],"domain_scores_gemma":[0.999732,0.00001848076,0.0000581841,0.0001689262,0.000007850897,0.00001462337],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003531194,0.00006136485,0.01064156,0.00003663156,0.00001552876,0.000001651522,0.00007631043,0.000298518,0.9851225,0.0004577409,0.0002450281,0.003007865],"study_design_scores_gemma":[0.002470879,0.000875704,0.04423169,0.0003955559,0.00002882978,0.00003356646,0.001551142,0.01820731,0.9088151,0.003295965,0.01941273,0.0006815224],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9918563,0.001011889,0.005884248,0.0008160767,0.0001342542,0.0002768454,0.000005187345,0.000009780018,0.000005413912],"genre_scores_gemma":[0.998092,0.000540036,0.001231264,0.000009638907,0.00001860561,0.0000647308,0.00002033237,0.000006502805,0.00001686972],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07630739,"threshold_uncertainty_score":0.3108679,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008665639442513683,"score_gpt":0.2053647972769465,"score_spread":0.1966991578344328,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}