{"id":"W2004949070","doi":"10.3389/fbioe.2014.00067","title":"The Induction of Recombinant Protein Bodies in Different Subcellular Compartments Reveals a Cryptic Plastid-Targeting Signal in the 27-kDa γ-Zein Sequence","year":2014,"lang":"en","type":"article","venue":"Frontiers in Bioengineering and Biotechnology","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"Regione Lombardia; Austrian Science Fund","keywords":"Plastid; Endoplasmic reticulum; ER retention; Recombinant DNA; Fusion protein; Biogenesis; Signal peptide; Cell biology; Biology; Protein targeting; Organelle; Subcellular localization; Transit Peptide; Protein Sorting Signals; Protein subcellular localization prediction; Biochemistry; Membrane protein; Chloroplast; Cytoplasm; Gene; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002446677,0.0003594154,0.0002633548,0.0001796533,0.000114802,0.0002723893,0.0002413961,0.0002591363,0.000510113],"category_scores_gemma":[0.0001346494,0.0001948375,0.0003282201,0.0001863542,0.0003304254,0.000283829,0.000383911,0.0006821065,0.0003758997],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003773396,"about_ca_system_score_gemma":0.0002423273,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002675563,"about_ca_topic_score_gemma":0.0003642243,"domain_scores_codex":[0.9998438,0.00002434375,0.00002080349,0.00004440242,0.00003729898,0.00002918061],"domain_scores_gemma":[0.9998074,0.00005196814,0.00006341466,0.00003230316,0.00001651921,0.00002835479],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001907034,0.000004309599,0.00004655809,0.00001017559,0.000001434074,0.00001273161,0.000006050172,0.00001961198,0.9996119,0.00007667929,0.000002666168,0.0001887638],"study_design_scores_gemma":[0.000003436382,0.00003272445,0.0004696743,0.000001593823,0.000005030556,0.00005488845,0.000006717109,0.0001959696,0.9985818,0.00002011522,0.0006267045,0.000001380077],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9813774,0.0004508699,0.01677888,0.000069991,0.00001928558,0.00005473335,0.0002591028,0.0001151846,0.0008744439],"genre_scores_gemma":[0.9821293,0.0004898736,0.01338625,0.00004057333,0.000005804398,0.00005490415,0.001012478,0.0000741676,0.002806591],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000510113,"threshold_uncertainty_score":0.00273782,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008665639442513683,"score_gpt":0.2053647972769465,"score_spread":0.1966991578344328,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}