{"id":"W2005195616","doi":"10.1042/bj20040226","title":"Protein interaction quantified <i>in vivo</i> by spectrally resolved fluorescence resonance energy transfer","year":2004,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":79,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Cancer Institute; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Förster resonance energy transfer; Yellow fluorescent protein; Acceptor; Chemistry; Fluorescence; Photobleaching; Green fluorescent protein; Biophysics; Population; Fluorescence recovery after photobleaching; Quenching (fluorescence); Saccharomyces cerevisiae; Yeast; Biochemistry; Biology; Membrane; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001714268,0.0002471488,0.0001969277,0.000065516,0.00008046527,0.00005474531,0.0003815574,0.000268225,0.00002142498],"category_scores_gemma":[0.00007656153,0.0002390425,0.0001237155,0.0001592131,0.0001558413,0.00002084944,0.00005190404,0.0004123259,0.000002852359],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001313732,"about_ca_system_score_gemma":0.0001183737,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000360812,"about_ca_topic_score_gemma":0.00002198104,"domain_scores_codex":[0.9983695,0.00005333182,0.0004358165,0.0004658941,0.0002212395,0.0004542125],"domain_scores_gemma":[0.9994243,0.000006238666,0.00007631606,0.0002782623,0.00008243709,0.0001324658],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004786097,0.0001541275,0.00004644935,0.000006928305,0.000008171952,0.00002517456,0.0000184342,0.00000567994,0.9926428,0.0001562715,0.005553426,0.0009039128],"study_design_scores_gemma":[0.0008470491,0.0002419918,0.00001983735,0.0001865496,0.000004179392,0.0001307925,0.00001986188,0.000006475669,0.970328,0.0004230862,0.02751302,0.0002791715],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8201132,0.002815506,0.1756587,0.00064089,0.0001534395,0.0002691774,0.00003317635,0.00005292215,0.0002628838],"genre_scores_gemma":[0.9690531,0.0009545455,0.02899208,0.0003944567,0.0002684119,0.00003531442,0.00003358378,0.00004601915,0.0002224322],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1489399,"threshold_uncertainty_score":0.974787,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007035124932107925,"score_gpt":0.2533806550280135,"score_spread":0.2463455300959056,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}