{"id":"W2005233992","doi":"10.1016/j.febslet.2007.05.002","title":"A single amino acid in the PSPG‐box plays an important role in the catalytic function of CaUGT2 (Curcumin glucosyltransferase), a Group D Family 1 glucosyltransferase from <i>Catharanthus roseus</i>","year":2007,"lang":"en","type":"article","venue":"FEBS Letters","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"RIKEN; Leibniz-Gemeinschaft; Leibniz-Institut für Pflanzenbiochemie; Japan Food Chemical Research Foundation; Kitasato University; University of British Columbia","keywords":"Catharanthus roseus; Glucosyltransferase; Biochemistry; Mutagenesis; Site-directed mutagenesis; Cysteine; Enzyme; Amino acid; Curcumin; Chemistry; Biology; Mutation; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001410352,0.0005811689,0.0004421927,0.0001273778,0.0002268229,0.0001891066,0.000248831,0.0004722146,0.001255591],"category_scores_gemma":[0.0001924274,0.0001617271,0.0004823807,0.0001513075,0.0004387255,0.0002553375,0.0002551217,0.0005987304,0.0009768876],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003309286,"about_ca_system_score_gemma":0.0003610508,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006175266,"about_ca_topic_score_gemma":0.0006549734,"domain_scores_codex":[0.9999146,0.0000178295,0.00000784373,0.00003022832,0.00001593542,0.00001350276],"domain_scores_gemma":[0.9998243,0.00004623808,0.00003767319,0.00002493068,0.00001144298,0.00005544095],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001943487,0.00001733389,0.0002981945,0.00004547225,0.000006019474,0.0002383985,0.00001545978,0.00009437726,0.997516,0.0001312537,0.0000352253,0.001407846],"study_design_scores_gemma":[0.00005057566,0.0002133738,0.007186691,0.00001483788,0.00003292437,0.001444465,0.00003572999,0.001223626,0.9861853,0.0001336619,0.003463361,0.00001554813],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.988417,0.0007151152,0.009330155,0.0001296509,0.00005854271,0.00003915247,0.0003267447,0.000161099,0.0008225911],"genre_scores_gemma":[0.9904069,0.0002672956,0.007307243,0.00008956406,0.0000155362,0.00003128829,0.0007867148,0.00004727101,0.001048128],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001255591,"threshold_uncertainty_score":0.004200399,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008629424810764248,"score_gpt":0.201677566543188,"score_spread":0.1930481417324238,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}