{"id":"W2006378088","doi":"10.1016/j.abb.2006.09.016","title":"Trp-49 of the family 3 β-glucosidase from Aspergillus niger is important for its transglucosidic activity: Creation of novel β-glucosidases with low transglucosidic efficiencies","year":2006,"lang":"en","type":"article","venue":"Archives of Biochemistry and Biophysics","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Enzyme kinetics; Aspergillus niger; Chemistry; Enzyme; Hydrolysis; Stereochemistry; Glucosidases; Biochemistry; Active site","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005145958,0.0002114218,0.0002631353,0.00002875319,0.00008254959,0.000009596866,0.0001668284,0.0001021974,0.000002089489],"category_scores_gemma":[0.00001773584,0.0001578961,0.0001727068,0.0001394823,0.0004308316,0.00001234547,0.00003114458,0.00006432601,5.519918e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003955366,"about_ca_system_score_gemma":0.00009567041,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000453721,"about_ca_topic_score_gemma":0.000005085988,"domain_scores_codex":[0.9989353,0.00002116015,0.0003475004,0.0003675262,0.0001698377,0.000158669],"domain_scores_gemma":[0.9991393,0.00004095997,0.0003640498,0.0003148644,0.0001014455,0.00003939229],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005256907,0.0005095165,0.0006236847,0.0002948825,0.00007119322,1.812812e-7,0.0001528005,0.0000406573,0.9967468,0.00004248348,0.00001544677,0.0009766368],"study_design_scores_gemma":[0.001142016,0.0002568752,0.009864205,0.0001368052,0.00009740127,0.000003509236,0.00009989953,0.000340292,0.98773,0.00005530412,0.00008939299,0.0001842667],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9921881,0.0008527105,0.005340165,0.0001355614,0.00003543809,0.0002847057,0.001055678,0.00000684035,0.0001008288],"genre_scores_gemma":[0.9986319,0.0003223865,0.0004198053,0.00003990515,0.0001149129,0.00001956063,0.0003238374,0.00001921707,0.0001084916],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009240519,"threshold_uncertainty_score":0.6438817,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006386079908213017,"score_gpt":0.2069949111558839,"score_spread":0.2006088312476709,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}