{"id":"W2006783687","doi":"10.1016/j.plaphy.2010.10.010","title":"Site-directed mutagenesis of histidine 69 and glutamic acid 148 alters the ribonuclease activity of pea ABR17 (PR10.4)","year":2011,"lang":"en","type":"article","venue":"Plant Physiology and Biochemistry","topic":"Legume Nitrogen Fixing Symbiosis","field":"Agricultural and Biological Sciences","cited_by":19,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; PrioNet Canada; Alberta Heritage Foundation for Medical Research; University of Alberta","keywords":"Ribonuclease; RNase P; Site-directed mutagenesis; Mutant; Histidine; S-tag; Mutagenesis; Biochemistry; Biology; Molecular biology; Amino acid; Escherichia coli; Chemistry; Gene; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002806128,0.0006921398,0.0003724645,0.0002357091,0.0001945679,0.0003358896,0.0005473412,0.0004805026,0.003051088],"category_scores_gemma":[0.0002895131,0.0002710685,0.0004808112,0.0001854182,0.0004910057,0.0001942669,0.0003454789,0.0007772441,0.001060502],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003579702,"about_ca_system_score_gemma":0.0003165923,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001090513,"about_ca_topic_score_gemma":0.001313178,"domain_scores_codex":[0.9997928,0.00004982892,0.00003049538,0.00003538241,0.00004336463,0.00004818382],"domain_scores_gemma":[0.9996773,0.00008160278,0.0001115997,0.00003327882,0.00002214633,0.00007406375],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004237682,0.00007456763,0.0002023812,0.00005276273,0.00001714506,0.0002724976,0.00003026215,0.000164184,0.9974278,0.0002041477,0.0000516575,0.001078898],"study_design_scores_gemma":[0.0001586558,0.0005361965,0.00387291,0.00002246339,0.00006280834,0.001124982,0.00008499913,0.001180482,0.9900516,0.0001131362,0.002763517,0.00002833257],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9957948,0.0001738604,0.002683417,0.00007438155,0.00006388572,0.00004311233,0.0002762276,0.000081199,0.0008092588],"genre_scores_gemma":[0.9951539,0.0001556542,0.002783932,0.00007691382,0.00001097466,0.00001871148,0.0004059131,0.00008077081,0.001313136],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003051088,"threshold_uncertainty_score":0.01020694,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01308015511956615,"score_gpt":0.1788550209041956,"score_spread":0.1657748657846295,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}