{"id":"W2006788743","doi":"10.3791/1144","title":"Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons","year":2009,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Simon Fraser University","keywords":"Live cell imaging; Green fluorescent protein; Photobleaching; Cell biology; Fluorescence recovery after photobleaching; Transfection; Biology; Fluorescence-lifetime imaging microscopy; Fluorescence microscope; Biophysics; Cell; Fluorescence; Cell culture; Optics; Physics; Membrane; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002933583,0.0004650761,0.0006618191,0.0004188394,0.0004578295,0.0005936541,0.0009248833,0.0004859468,0.0009482996],"category_scores_gemma":[0.0001533258,0.0002898819,0.000451905,0.0003419612,0.0003330476,0.0004312593,0.0004179422,0.0009766161,0.001115141],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001128816,"about_ca_system_score_gemma":0.0006531451,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.006722244,"about_ca_topic_score_gemma":0.01612912,"domain_scores_codex":[0.9996873,0.00001291935,0.00003062681,0.00009731435,0.0001205517,0.00005122285],"domain_scores_gemma":[0.999874,0.00003185413,0.00001989358,0.00001592217,0.00003314309,0.00002511968],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002028025,0.00001604365,0.0001932043,0.0000532109,0.000005494668,0.00007064057,0.00003806356,0.0001451265,0.9979377,0.00009817812,0.00005373098,0.001368255],"study_design_scores_gemma":[0.00001163349,0.000130029,0.006211248,0.00002739887,0.00002441444,0.0002973999,0.00007872035,0.003050844,0.9879285,0.0001176019,0.002109936,0.00001231503],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.831574,0.009505326,0.1323613,0.0004111832,0.0003924795,0.0006414048,0.009103283,0.001331509,0.01467948],"genre_scores_gemma":[0.7846863,0.007146444,0.1913949,0.0004180865,0.00008575597,0.0005395942,0.003783203,0.000342259,0.01160336],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.006722244,"threshold_uncertainty_score":0.01336622,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02363383796230945,"score_gpt":0.363818192175073,"score_spread":0.3401843542127636,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}