{"id":"W2007263952","doi":"10.1016/s0165-0270(99)00148-x","title":"Digital analysis of light microscope immunofluorescence: high-resolution co-localization of synaptic proteins in cultured neurons","year":2000,"lang":"en","type":"article","venue":"Journal of Neuroscience Methods","topic":"Photoreceptor and optogenetics research","field":"Neuroscience","cited_by":15,"is_retracted":false,"has_abstract":false,"ca_institutions":"Institute for Biological Sciences","funders":"","keywords":"Immunofluorescence; Resolution (logic); Microscope; Biology; Cell biology; Neuroscience; Biophysics; Optics; Physics; Computer science; Artificial intelligence; Antibody","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003114167,0.0003995807,0.0003232133,0.001695473,0.0005908154,0.0008044649,0.0007273656,0.0004247352,0.01282545],"category_scores_gemma":[0.0004715653,0.000339454,0.0003182249,0.0009193413,0.0004350502,0.0005089071,0.0004533873,0.0008489778,0.001612237],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007789796,"about_ca_system_score_gemma":0.0006376602,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001367864,"about_ca_topic_score_gemma":0.002652887,"domain_scores_codex":[0.9998168,0.0000104805,0.00002040924,0.00004226849,0.00007287721,0.00003718791],"domain_scores_gemma":[0.9995357,0.0001300238,0.00003942733,0.0001010469,0.0001462756,0.00004755608],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002020546,0.00007576548,0.001351297,0.0002294354,0.00002404766,0.0001918731,0.0001298245,0.00057327,0.952459,0.001748743,0.001241519,0.04177318],"study_design_scores_gemma":[0.00004363719,0.0001073569,0.02930988,0.00005075794,0.00006969042,0.001008471,0.0002064882,0.04089449,0.9161367,0.001658117,0.01046506,0.00004926112],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5675059,0.0007972297,0.4053704,0.000675361,0.0003557703,0.0002859328,0.004559959,0.007738336,0.01271112],"genre_scores_gemma":[0.5926903,0.001130738,0.3899356,0.0002790609,0.00009879748,0.0009009105,0.00263898,0.001706661,0.0106189],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.01282545,"threshold_uncertainty_score":0.04290545,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04173733105476009,"score_gpt":0.3880555333296006,"score_spread":0.3463182022748405,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}