{"id":"W2007388934","doi":"10.1016/j.pep.2005.10.006","title":"Intracellular production of a soluble and functional monomeric streptavidin in Escherichia coli and its application for affinity purification of biotinylated proteins","year":2005,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Biotinylation; Streptavidin; Agarose; Biotin; Avidin; Affinity chromatography; Biochemistry; Chemistry; Escherichia coli; Chromatography; Sepharose; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003702706,0.0004731631,0.0006190367,0.0002759189,0.0002602887,0.0005590597,0.0003752289,0.0005147691,0.0006855534],"category_scores_gemma":[0.0002802687,0.0002843482,0.0004984625,0.0004271277,0.000278452,0.0002781715,0.0004132854,0.00109961,0.0008737177],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000608768,"about_ca_system_score_gemma":0.0004387194,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00183557,"about_ca_topic_score_gemma":0.00209795,"domain_scores_codex":[0.9997953,0.00003788422,0.00002127956,0.00003565307,0.00006317649,0.00004675],"domain_scores_gemma":[0.9998074,0.00004373294,0.00002553198,0.00003283966,0.00004659585,0.00004396016],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002907104,0.00001568956,0.00004161983,0.00003554203,0.000003732384,0.00001938023,0.00001640574,0.00003379901,0.9986603,0.0001894822,0.00002843506,0.0009266548],"study_design_scores_gemma":[0.00000601021,0.00004891523,0.000272821,0.000002755319,0.000008757932,0.00006900878,0.00001342524,0.0004891024,0.9975274,0.00003374692,0.001524441,0.000003426965],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8688989,0.002847649,0.1230705,0.0005694007,0.0001353166,0.0002266581,0.001050532,0.0004428244,0.002758233],"genre_scores_gemma":[0.933315,0.001526298,0.05445987,0.0001193109,0.0000268177,0.0001440671,0.002308798,0.0001288183,0.00797109],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00183557,"threshold_uncertainty_score":0.004416943,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01408755897040468,"score_gpt":0.231951768857267,"score_spread":0.2178642098868623,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}