{"id":"W2007677251","doi":"10.1038/nchembio.191","title":"Designer enzymes for glycosphingolipid synthesis by directed evolution","year":2009,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":83,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"Royal Society","keywords":"Glycosphingolipid; Synthetic biology; Mutagenesis; Enzyme; Computational biology; Rational design; Directed evolution; Biochemistry; Mutant; Protein engineering; Homogeneous; Combinatorial chemistry; Chemistry; Biology; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007316871,0.0005857423,0.0005422428,0.0003518172,0.0002756563,0.0008811968,0.0006704636,0.0005987301,0.0008880689],"category_scores_gemma":[0.0006220781,0.0004407304,0.0003363621,0.0004424105,0.0004268019,0.0005538928,0.0006075348,0.001524119,0.0006159366],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009879208,"about_ca_system_score_gemma":0.0003508257,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004577959,"about_ca_topic_score_gemma":0.0009440396,"domain_scores_codex":[0.9997551,0.00004552884,0.00002147064,0.00005902757,0.00007531792,0.00004357953],"domain_scores_gemma":[0.9998399,0.00004702351,0.00002601716,0.00003577265,0.00002577777,0.00002556161],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004080794,0.0001156429,0.001268462,0.0003563027,0.0000765642,0.0002485255,0.0002233923,0.007420099,0.8573648,0.02892922,0.001399641,0.1021893],"study_design_scores_gemma":[0.0001070051,0.0003051756,0.0004568526,0.00003709683,0.0000833825,0.0003243164,0.00004891713,0.01493414,0.9349244,0.004619702,0.04410976,0.00004925558],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.688524,0.01366745,0.272533,0.001070353,0.0005117087,0.0003368768,0.0007519293,0.002438436,0.02016636],"genre_scores_gemma":[0.8309979,0.007935117,0.1511123,0.0003460883,0.00004595152,0.0001813879,0.0006066562,0.0003719703,0.008402538],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009879208,"threshold_uncertainty_score":0.007167876,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005773284049939455,"score_gpt":0.2380585707413475,"score_spread":0.232285286691408,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}