{"id":"W2007677251","doi":"10.1038/nchembio.191","title":"Designer enzymes for glycosphingolipid synthesis by directed evolution","year":2009,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":83,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"Royal Society","keywords":"Glycosphingolipid; Synthetic biology; Mutagenesis; Enzyme; Computational biology; Rational design; Directed evolution; Biochemistry; Mutant; Protein engineering; Homogeneous; Combinatorial chemistry; Chemistry; Biology; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","research_integrity"],"consensus_categories":[],"category_scores_codex":[0.0001130367,0.000302701,0.0004089201,0.00002902166,0.0000895615,0.00002092815,0.00036746,0.001670288,0.0005172997],"category_scores_gemma":[0.001829945,0.0002667944,0.0002336485,0.0001480311,0.0001169202,0.00004775157,0.00003042896,0.0005488864,0.00001008411],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001739503,"about_ca_system_score_gemma":0.00003591713,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005147908,"about_ca_topic_score_gemma":3.662186e-7,"domain_scores_codex":[0.9984853,0.00001997002,0.0002990716,0.0005947031,0.0001029142,0.0004980249],"domain_scores_gemma":[0.9984231,0.0008585691,0.0001142343,0.0003531937,0.0001055854,0.0001453259],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002145055,0.0001092231,0.00005888499,0.00004143832,0.00004441549,0.000001072725,0.000009775808,1.643384e-7,0.9734209,0.0008302839,0.01851204,0.006757289],"study_design_scores_gemma":[0.0003083988,0.00001508559,0.000008996059,0.00002738642,0.00006033288,0.00001044182,0.00001080885,0.00008385009,0.961563,0.002749554,0.03483935,0.0003228141],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9683487,0.0089652,0.0005460978,0.002905268,0.0002567864,0.0002141454,0.0006883699,0.000938987,0.01713646],"genre_scores_gemma":[0.9971942,0.00004387775,0.0009160159,0.0003625893,0.0006001398,0.00008057776,0.000359682,0.00002304762,0.0004198134],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02884557,"threshold_uncertainty_score":0.9999784,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005773284049939455,"score_gpt":0.2380585707413475,"score_spread":0.232285286691408,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}