{"id":"W2007993460","doi":"10.1186/1756-0500-3-126","title":"An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L","year":2010,"lang":"en","type":"article","venue":"BMC Research Notes","topic":"Plant Gene Expression Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":59,"is_retracted":false,"has_abstract":true,"ca_institutions":"Nova Scotia Department of Agriculture","funders":"Temasek Life Sciences Laboratory","keywords":"Jatropha curcas; RNA; RNA extraction; Complementary DNA; cDNA library; Jatropha; Biology; Biochemistry; Gene; Biotechnology; Biodiesel","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005948495,0.001447416,0.0008035325,0.001345218,0.0006101243,0.0005472745,0.000825571,0.0006365624,0.001871075],"category_scores_gemma":[0.0004713963,0.0005891353,0.001005201,0.0009178962,0.0003832794,0.000458837,0.000495074,0.001498731,0.002618875],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000381977,"about_ca_system_score_gemma":0.0008849216,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001205421,"about_ca_topic_score_gemma":0.002642942,"domain_scores_codex":[0.9990628,0.0001034783,0.0001320426,0.0002643369,0.000355794,0.00008153396],"domain_scores_gemma":[0.9996125,0.00006807272,0.00006964683,0.00007888678,0.0001292437,0.00004162738],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001515462,0.00001137799,0.00006532793,0.0001000419,0.000004942601,0.00005618755,0.00001373473,0.00003057794,0.9969304,0.00004206171,0.0001141452,0.002615968],"study_design_scores_gemma":[0.00003722594,0.0002930925,0.005934455,0.00004355799,0.0001275293,0.00152522,0.00004140795,0.001131654,0.9537724,0.0001264285,0.03689541,0.00007152886],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2651143,0.01181339,0.6871297,0.001054661,0.001068222,0.004063918,0.01172852,0.005728661,0.01229862],"genre_scores_gemma":[0.2291034,0.008739711,0.6908132,0.0007925776,0.0002937409,0.004656691,0.03699976,0.001252994,0.0273479],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001871075,"threshold_uncertainty_score":0.006259382,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03052846594376914,"score_gpt":0.3756176249394784,"score_spread":0.3450891589957093,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}