{"id":"W2007993460","doi":"10.1186/1756-0500-3-126","title":"An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L","year":2010,"lang":"en","type":"article","venue":"BMC Research Notes","topic":"Plant Gene Expression Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":59,"is_retracted":false,"has_abstract":true,"ca_institutions":"Nova Scotia Department of Agriculture","funders":"Temasek Life Sciences Laboratory","keywords":"Jatropha curcas; RNA; RNA extraction; Complementary DNA; cDNA library; Jatropha; Biology; Biochemistry; Gene; Biotechnology; Biodiesel","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000451076,0.00009034827,0.0001360768,0.0001134335,0.00009414431,0.00005238227,0.0001696479,0.0002309645,0.00004233527],"category_scores_gemma":[0.000545394,0.00007771748,0.00006433162,0.0001190766,0.0001125838,0.00002071422,0.0001014016,0.0001914124,8.29212e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000002278455,"about_ca_system_score_gemma":0.0001050426,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001027058,"about_ca_topic_score_gemma":0.00009157322,"domain_scores_codex":[0.9989784,0.0002094077,0.0001619738,0.0003249218,0.0001472107,0.0001780495],"domain_scores_gemma":[0.9991227,0.0002067719,0.00007637763,0.0003435162,0.0001470903,0.000103563],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004299793,0.00002394786,0.007948489,0.00002018386,0.00003243939,1.924057e-7,0.00002713127,0.000007396218,0.9846751,0.00005220829,0.0007193863,0.006063535],"study_design_scores_gemma":[0.000406153,0.0001660635,0.005257855,0.000007469998,0.00001607053,0.000003385884,0.00008257988,0.01654536,0.9746193,0.0008457839,0.001960115,0.00008991241],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.944813,0.0001846615,0.05422558,0.0001350916,0.00007380663,0.0002232217,0.0002437526,0.00001064691,0.00009029674],"genre_scores_gemma":[0.7531602,0.00007234587,0.2457784,0.00001635113,0.0002726893,0.00001917763,0.0005618171,0.00001470244,0.000104232],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1916527,"threshold_uncertainty_score":0.3169227,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03052846594376914,"score_gpt":0.3756176249394784,"score_spread":0.3450891589957093,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}