{"id":"W2008177395","doi":"10.1038/nprot.2013.105","title":"Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli","year":2013,"lang":"en","type":"article","venue":"Nature Protocols","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":183,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Synthetic biology; Biology; Computational biology; Gene; Function (biology); Escherichia coli; Functional genomics; Plasmid; Gene expression; Regulation of gene expression; Metabolic engineering; Transfer RNA; Genetics; Genomics; RNA; Genome","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000137609,0.0001205677,0.0001488116,0.00004439844,0.00001576538,0.00002009998,0.00009946438,0.0002731189,0.000007437742],"category_scores_gemma":[0.0001083444,0.0001067728,0.00002670732,0.00006188927,0.00002222249,0.000003669488,0.00005534858,0.0001407574,0.000001559932],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007139842,"about_ca_system_score_gemma":0.00002573967,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007112578,"about_ca_topic_score_gemma":0.000003683829,"domain_scores_codex":[0.9992995,0.00005865011,0.0001679245,0.0002359837,0.00007754449,0.0001604492],"domain_scores_gemma":[0.9995245,0.00002499391,0.00004173307,0.0002641628,0.00006563015,0.00007903357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001065096,0.0000299563,0.000922095,0.00004051007,0.000007695829,8.52299e-7,0.00002659649,0.002247231,0.9952217,0.000005986283,0.0003313936,0.001059487],"study_design_scores_gemma":[0.0006578438,0.0001946275,0.01354422,0.00009429735,0.000003808163,0.00000232325,0.000004649298,0.0004641715,0.9815586,0.00002928912,0.003319429,0.0001267286],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8627797,0.0003208052,0.09301361,0.00007985721,0.00002950263,0.04374227,0.000004009342,0.00001044164,0.00001978523],"genre_scores_gemma":[0.9259978,0.000005328353,0.04417899,0.0001864429,0.00004137843,0.02948394,0.000002737424,0.00002067857,0.0000827176],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06321808,"threshold_uncertainty_score":0.435407,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01108039518141564,"score_gpt":0.2846392700289164,"score_spread":0.2735588748475007,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}