{"id":"W2008194805","doi":"10.1016/j.pep.2007.03.011","title":"Tri-cistronic cloning, overexpression and purification of human Rad9, Rad1, Hus1 protein complex","year":2007,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"DNA Repair Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Canadian Institutes of Health Research; Canada Research Chairs","keywords":"Schizosaccharomyces pombe; Cloning (programming); DNA; Biology; Saccharomyces cerevisiae; Molecular biology; Cell biology; Yeast; Biochemistry; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007276667,0.0002168462,0.0002021518,0.000100845,0.0002184637,0.00003622509,0.0001785774,0.0002676971,0.00003850166],"category_scores_gemma":[0.0001008811,0.0002011963,0.00005570427,0.00009522545,0.0001293525,0.00002170016,0.0001114458,0.0001375016,0.000002580767],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001858003,"about_ca_system_score_gemma":0.00003262235,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001705973,"about_ca_topic_score_gemma":0.000002802806,"domain_scores_codex":[0.9983022,0.0001417504,0.0004773209,0.0005597678,0.0002564115,0.0002625166],"domain_scores_gemma":[0.9988179,0.00001215264,0.0004080334,0.0005302578,0.0001152903,0.000116362],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001985603,0.0001142794,0.000153018,0.0001105231,0.000008921036,4.339588e-7,0.000110477,9.42276e-7,0.9889445,0.001958846,0.000139297,0.008260211],"study_design_scores_gemma":[0.0009427954,0.0003004825,0.003664947,0.0002047756,0.000009847098,0.000005989079,0.0001083267,0.00006971366,0.9845666,0.0007384202,0.009168174,0.0002199201],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9869452,0.001227813,0.009565965,0.00009599658,0.00002629834,0.001381417,0.00001319718,0.00004122378,0.0007028473],"genre_scores_gemma":[0.9903476,0.00006125018,0.008102887,0.0000218093,0.00008577042,0.0001606166,0.000262151,0.00003073204,0.0009271404],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009028877,"threshold_uncertainty_score":0.8204545,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01937013209300504,"score_gpt":0.2778443122418039,"score_spread":0.2584741801487989,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}