{"id":"W2008194805","doi":"10.1016/j.pep.2007.03.011","title":"Tri-cistronic cloning, overexpression and purification of human Rad9, Rad1, Hus1 protein complex","year":2007,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"DNA Repair Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Canadian Institutes of Health Research; Canada Research Chairs","keywords":"Schizosaccharomyces pombe; Cloning (programming); DNA; Biology; Saccharomyces cerevisiae; Molecular biology; Cell biology; Yeast; Biochemistry; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006350151,0.0006594248,0.0006777644,0.0006297856,0.0006231388,0.0008364477,0.0008298351,0.0003821367,0.002213636],"category_scores_gemma":[0.0004355322,0.0004830859,0.0006555682,0.001123627,0.000354916,0.0005254423,0.0007469318,0.001615496,0.002105964],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009195787,"about_ca_system_score_gemma":0.0005417743,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00100694,"about_ca_topic_score_gemma":0.001874428,"domain_scores_codex":[0.999314,0.00009740352,0.0001107094,0.0001948588,0.0001636678,0.0001194668],"domain_scores_gemma":[0.99955,0.0001004463,0.0000638348,0.0001456763,0.00005810018,0.00008178684],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000248161,0.00009806186,0.0002870776,0.0001055962,0.00001629591,0.00008741113,0.00007426428,0.0001493576,0.9946043,0.0007880729,0.0005586925,0.002982731],"study_design_scores_gemma":[0.00003382294,0.00009523086,0.002969394,0.00001306339,0.0000349173,0.0002475987,0.00004640544,0.001678091,0.9815933,0.0002457952,0.01302595,0.0000165677],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8356575,0.005030024,0.1244785,0.001404244,0.0009500041,0.0008099974,0.02165461,0.001924057,0.008091039],"genre_scores_gemma":[0.7800038,0.003227985,0.1066821,0.0003897212,0.0001566659,0.0006186284,0.05866498,0.0007390764,0.04951708],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002213636,"threshold_uncertainty_score":0.007405341,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01937013209300504,"score_gpt":0.2778443122418039,"score_spread":0.2584741801487989,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}