{"id":"W2008477360","doi":"10.1074/jbc.m305200200","title":"Deletions of Any Single Residues in Glu40-Ser48 Loop Connecting A Domain and the First Transmembrane Helix of Sarcoplasmic Reticulum Ca2+-ATPase Result in Almost Complete Inhibition of Conformational Transition and Hydrolysis of Phosphoenzyme Intermediate","year":2003,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Genetic and rare skin diseases.","field":"Biochemistry, Genetics and Molecular Biology","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"University of Toronto; Ministry of Education, Culture, Sports, Science and Technology","keywords":"Transmembrane domain; Endoplasmic reticulum; ATP hydrolysis; Mutagenesis; Chemistry; Mutant; Conformational change; Biophysics; ATPase; Stereochemistry; Helix (gastropod); Transmembrane protein; Loop (graph theory); Crystallography; Biochemistry; Enzyme; Biology; Membrane; Gene; Receptor","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004988608,0.0001057426,0.0003487427,0.0000434083,0.00002374737,0.000003536247,0.00007272333,0.0001501107,0.00001815564],"category_scores_gemma":[0.0003092737,0.00007434552,0.0001056553,0.00011803,0.0005024284,0.000007136604,0.00002007856,0.0001134967,2.41626e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001085688,"about_ca_system_score_gemma":0.00004392371,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002353129,"about_ca_topic_score_gemma":0.00002911944,"domain_scores_codex":[0.9987588,0.00009953369,0.0007966973,0.0001199532,0.0001174783,0.0001075533],"domain_scores_gemma":[0.9991014,0.0001201826,0.0004977867,0.00009050332,0.0001446122,0.00004551703],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001169485,0.0001899102,0.003164514,0.0002262365,0.00003934122,0.00001109244,0.0003300816,0.0002977782,0.9944739,0.00001725582,0.00000996838,0.00007046676],"study_design_scores_gemma":[0.003972114,0.0003203126,0.008695715,0.000334559,0.00003564742,0.0002308233,0.001279044,0.0001060136,0.9845721,0.0003267953,0.0000402624,0.00008657941],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973847,0.001894946,0.0002252492,0.0001888342,0.00001180977,0.00009614337,0.0001059233,7.875989e-7,0.00009160676],"genre_scores_gemma":[0.998791,0.0008137997,0.0002885443,0.00002675627,0.0000146254,0.000002376978,0.00005731585,0.000004009926,0.000001503366],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009901737,"threshold_uncertainty_score":0.3031722,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008445054236799907,"score_gpt":0.2065050609506746,"score_spread":0.1980600067138747,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}