{"id":"W2008834181","doi":"10.1016/j.abb.2005.09.013","title":"Trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the Aspergillus niger family 3 β-glucosidase: Substitution results in enzymes with mainly transglucosidic activity","year":2005,"lang":"en","type":"article","venue":"Archives of Biochemistry and Biophysics","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Chemistry; Cellobiose; Aspergillus niger; Adduct; Stereochemistry; Acceptor; Residue (chemistry); Hydrolysis; Enzyme; Substrate (aquarium); Glucosidases; Cleavage (geology); Organic chemistry; Biochemistry; Cellulase; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002286911,0.001410073,0.0006340016,0.0002318999,0.0004471526,0.0006092135,0.0006434856,0.0009664638,0.004410233],"category_scores_gemma":[0.0004595676,0.000325831,0.0006505855,0.0002539972,0.0006400052,0.0004955376,0.0004876565,0.001161737,0.002581674],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004295961,"about_ca_system_score_gemma":0.0004521154,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000668372,"about_ca_topic_score_gemma":0.0004207481,"domain_scores_codex":[0.9997407,0.00005853946,0.00004105753,0.00007029734,0.00005243846,0.00003688873],"domain_scores_gemma":[0.9993715,0.0001013181,0.0001794757,0.00007473076,0.00004857845,0.0002244495],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008021582,0.00007156068,0.0004502513,0.00007082965,0.00002622997,0.001317673,0.00003081029,0.0004425091,0.994302,0.0003239277,0.0001235756,0.002038414],"study_design_scores_gemma":[0.0001181113,0.0003089125,0.003968026,0.00001898426,0.00007003184,0.00307013,0.00007152889,0.001955124,0.9876,0.00017364,0.002593817,0.00005173545],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9851365,0.0006438824,0.01033732,0.0002720176,0.0002797563,0.00006779136,0.0007890517,0.0003285351,0.002145057],"genre_scores_gemma":[0.9943295,0.0002241072,0.002833599,0.00008487529,0.00003483261,0.00002972325,0.000682279,0.0001100947,0.001670957],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004410233,"threshold_uncertainty_score":0.0147537,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007268421835657185,"score_gpt":0.2110400499476007,"score_spread":0.2037716281119435,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}