{"id":"W2008834181","doi":"10.1016/j.abb.2005.09.013","title":"Trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the Aspergillus niger family 3 β-glucosidase: Substitution results in enzymes with mainly transglucosidic activity","year":2005,"lang":"en","type":"article","venue":"Archives of Biochemistry and Biophysics","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Chemistry; Cellobiose; Aspergillus niger; Adduct; Stereochemistry; Acceptor; Residue (chemistry); Hydrolysis; Enzyme; Substrate (aquarium); Glucosidases; Cleavage (geology); Organic chemistry; Biochemistry; Cellulase; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001268618,0.0001759788,0.000183726,0.00002348473,0.0001137625,0.00001219339,0.0001401723,0.00009629169,5.788075e-7],"category_scores_gemma":[0.00002901877,0.0001155063,0.00008443854,0.0001215252,0.0005064196,0.00001635889,0.00003458968,0.000120504,7.414961e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005784572,"about_ca_system_score_gemma":0.0000752188,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003186605,"about_ca_topic_score_gemma":0.00003291807,"domain_scores_codex":[0.9990928,0.00004782233,0.0002208013,0.0003607936,0.000117072,0.0001606748],"domain_scores_gemma":[0.9993542,0.00006535959,0.0001689074,0.0003384351,0.00003370682,0.00003943552],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001144405,0.0001703432,0.000397332,0.0001320075,0.00005065316,1.911415e-7,0.0003514059,0.00002237595,0.989615,0.00006091206,0.00001405734,0.008041338],"study_design_scores_gemma":[0.001385168,0.0001545258,0.0236939,0.00007931236,0.00005946052,0.000007440016,0.00006831877,0.00029399,0.9733406,0.00007059624,0.000704346,0.0001423482],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.995894,0.0008969205,0.0008634377,0.001688955,0.00002167435,0.0003081642,0.0002337415,0.000005032309,0.00008810875],"genre_scores_gemma":[0.9982057,0.001171493,0.000180255,0.0000909222,0.0001058539,0.00002364364,0.00005905642,0.00001214429,0.0001509888],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02329657,"threshold_uncertainty_score":0.4710208,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007268421835657185,"score_gpt":0.2110400499476007,"score_spread":0.2037716281119435,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}