{"id":"W2009170690","doi":"10.1089/hum.2006.17.353","title":"Insertional Mutagenesis at Positions 520 and 584 of Adeno-Associated Virus Type 2 (AAV2) Capsid Gene and Generation of AAV2 Vectors with Eliminated Heparin- Binding Ability and Introduced Novel Tropism","year":2006,"lang":"en","type":"article","venue":"Human Gene Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institutes of Health; National Institute of Allergy and Infectious Diseases; Cancer Research Society","keywords":"Tropism; Transduction (biophysics); Biology; Adeno-associated virus; Insertional mutagenesis; Capsid; Tissue tropism; Mutant; Mutagenesis; Gene; Virology; Molecular biology; Cell biology; Virus; Recombinant DNA; Vector (molecular biology); Genetics; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002568352,0.0006687951,0.0003484629,0.0002834687,0.0001588296,0.0002179915,0.0002858083,0.0003209598,0.001276301],"category_scores_gemma":[0.0002402005,0.0002918956,0.0003934891,0.0001909921,0.0002719985,0.0001374023,0.0002359069,0.0004750686,0.0005843288],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002872468,"about_ca_system_score_gemma":0.0003547526,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000644554,"about_ca_topic_score_gemma":0.001033646,"domain_scores_codex":[0.9997213,0.00006650553,0.00003871613,0.00006261903,0.000068594,0.00004208395],"domain_scores_gemma":[0.999798,0.00004684528,0.00008859137,0.00002233311,0.0000165784,0.00002775502],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006418801,0.00003082384,0.0001972595,0.00002106418,0.000005079703,0.00007642023,0.00001081432,0.00009196982,0.9980338,0.0001411587,0.00002084138,0.001306486],"study_design_scores_gemma":[0.00003762271,0.000392275,0.002671049,0.000007631704,0.00004308725,0.0007904334,0.00001448547,0.001030484,0.9918527,0.000080194,0.003064944,0.00001513191],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9800838,0.0004024423,0.01743631,0.0000646581,0.000055433,0.0001493685,0.0003022794,0.00008668267,0.001419064],"genre_scores_gemma":[0.9533471,0.0006833126,0.04004963,0.00009468033,0.00001969107,0.0001649864,0.001366678,0.0000803134,0.00419364],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001276301,"threshold_uncertainty_score":0.004269719,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02727639181272883,"score_gpt":0.2726479048005846,"score_spread":0.2453715129878558,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}