{"id":"W2009480004","doi":"10.1186/1471-2091-14-23","title":"A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding","year":2013,"lang":"en","type":"article","venue":"BMC Biochemistry","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"Ministry of Education, Culture, Sports, Science and Technology","keywords":"Residue (chemistry); Chitosanase; Biochemistry; Streptomycetaceae; Streptomyces; Stereochemistry; Arginine; Substrate specificity; Chemistry; Conserved sequence; Bacteria; Substrate (aquarium); Enzyme; Catalysis; Biology; Amino acid; Peptide sequence; Actinomycetales; Genetics; Ecology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006486951,0.0002502523,0.0002778982,0.00003047213,0.00008636645,0.00003101772,0.000289151,0.0001782259,0.00007735552],"category_scores_gemma":[0.0001229513,0.0001806113,0.0001379406,0.0001632597,0.0002949892,0.00000601258,0.0002539142,0.00009815004,0.00000294013],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001130628,"about_ca_system_score_gemma":0.0001067397,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001286966,"about_ca_topic_score_gemma":0.001617517,"domain_scores_codex":[0.9986484,0.00004349683,0.0003707447,0.0005087262,0.0001517459,0.0002768805],"domain_scores_gemma":[0.9990029,0.00006260532,0.0002161324,0.0005803806,0.00004704252,0.00009090543],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003668133,0.00004479774,0.3440963,0.00004929116,0.00006864677,0.000001397418,0.00002939704,9.168927e-7,0.6547013,0.00000211927,0.0008732867,0.00009588523],"study_design_scores_gemma":[0.0007627605,0.00003012832,0.1634955,0.00009817861,0.00003411218,0.000002168184,0.0008584719,0.00001158083,0.8344019,0.00003860792,0.00007109052,0.000195504],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9960867,0.002690676,0.000003682261,0.000349848,0.00005266106,0.0001736462,0.0003273805,0.00000965841,0.0003057577],"genre_scores_gemma":[0.9987286,0.0003284474,0.000232765,0.0001502106,0.0001391719,0.00004110253,0.0001508653,0.00002048097,0.0002083875],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1806008,"threshold_uncertainty_score":0.7365115,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01076702384658964,"score_gpt":0.219147601853355,"score_spread":0.2083805780067654,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}