{"id":"W2009743304","doi":"10.1038/nmeth.2305","title":"Single-molecule analysis of gene expression using two-color RNA labeling in live yeast","year":2012,"lang":"en","type":"article","venue":"Nature Methods","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":313,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal","funders":"National Institute of General Medical Sciences; Canadian Institutes of Health Research","keywords":"Gene expression; RNA; Live cell imaging; Messenger RNA; Biology; Yeast; Gene; Molecular biology; Cell biology; Cell; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002447818,0.0003828137,0.0004519372,0.0002588962,0.0002965932,0.000615804,0.0006476818,0.0004720672,0.0007365601],"category_scores_gemma":[0.0001957023,0.0002755081,0.0004251261,0.0002945817,0.0004908632,0.0005041956,0.0002883644,0.001213942,0.0005535893],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000604754,"about_ca_system_score_gemma":0.0002771219,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00138476,"about_ca_topic_score_gemma":0.002161049,"domain_scores_codex":[0.9998486,0.00001654238,0.000009480707,0.00005496437,0.00004712071,0.00002326126],"domain_scores_gemma":[0.9998412,0.00004974274,0.00002477011,0.00003064332,0.00002961458,0.00002397075],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000314803,0.000007060081,0.00005278902,0.00002288769,0.000002152033,0.00001085265,0.00001029751,0.0001006572,0.9987682,0.0001589389,0.00003139414,0.000803352],"study_design_scores_gemma":[0.000008308453,0.00002356669,0.00095408,0.000002377873,0.000004679477,0.00003132825,0.00001931679,0.003199226,0.9949445,0.0001242399,0.0006824357,0.000005958012],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7435567,0.001635093,0.2468471,0.0004809934,0.000187705,0.0001279433,0.002626797,0.001354293,0.003183347],"genre_scores_gemma":[0.859696,0.002111019,0.1304394,0.0002825864,0.00005084162,0.0003233882,0.001806648,0.0004962683,0.004793869],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00138476,"threshold_uncertainty_score":0.004387796,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03300937113626319,"score_gpt":0.3602103567085621,"score_spread":0.3272009855722989,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}