{"id":"W2009930207","doi":"10.1016/j.mcp.2005.06.001","title":"High-throughput qualitative multiplex 5′ nuclease assay using post-only PCR analysis","year":2005,"lang":"en","type":"article","venue":"Molecular and Cellular Probes","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"Saskatchewan Health; Saskatchewan Disease Control Laboratory","funders":"","keywords":"TaqMan; Biology; Multiplex; Computational biology; Nuclease; Identification (biology); Protocol (science); Throughput; Real-time polymerase chain reaction; Multiplex polymerase chain reaction; Molecular biology; Polymerase chain reaction; Genetics; DNA; Computer science; Pathology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001947738,0.002640466,0.002608152,0.001729209,0.0006528374,0.001419106,0.002456591,0.001025828,0.004446784],"category_scores_gemma":[0.002468211,0.001724255,0.001715357,0.001007584,0.0009527939,0.001284482,0.001287237,0.002632087,0.006530501],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007908748,"about_ca_system_score_gemma":0.001442613,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000682753,"about_ca_topic_score_gemma":0.001954617,"domain_scores_codex":[0.9964964,0.0005764668,0.0002027619,0.001260711,0.001087374,0.0003762276],"domain_scores_gemma":[0.9977643,0.000959604,0.0001933141,0.000386173,0.000531156,0.000165432],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002135788,0.0001589631,0.000404698,0.0002076218,0.00002991475,0.00004133967,0.00005021769,0.0003742081,0.9767159,0.0007508268,0.0008807291,0.02017211],"study_design_scores_gemma":[0.0000497704,0.0005190633,0.001711164,0.00003573454,0.0001100829,0.0002676564,0.00003050585,0.009247945,0.9789022,0.001274424,0.007785241,0.00006620026],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.02833239,0.0005900412,0.9623868,0.0001363451,0.0001945777,0.0006413026,0.001916797,0.003717191,0.002084478],"genre_scores_gemma":[0.07983156,0.0008310024,0.9015266,0.0003502365,0.0001494563,0.002198323,0.006364909,0.0006708312,0.008077197],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004446784,"threshold_uncertainty_score":0.01487595,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01151669240598321,"score_gpt":0.2906676891728206,"score_spread":0.2791509967668374,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}