{"id":"W2010477728","doi":"10.1042/bj20040863","title":"Random mutagenesis of bacterial luciferase: critical role of Glu175 in the control of luminescence decay","year":2005,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research","keywords":"Luciferases; Luciferase; Photobacterium phosphoreum; Photorhabdus luminescens; Biology; Bioluminescence; Photobacterium; Vibrio; Biochemistry; Mutagenesis; Mutant; Bacteria; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008293904,0.0001611385,0.0003553902,0.00007058253,0.00003865547,0.00001338018,0.000606095,0.0002276676,0.00007782738],"category_scores_gemma":[0.0006827939,0.0001145774,0.0002216638,0.0001953651,0.0005352054,0.00001258213,0.00006876238,0.0002449081,0.000001313556],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001350612,"about_ca_system_score_gemma":0.0001693521,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001146864,"about_ca_topic_score_gemma":0.000004158518,"domain_scores_codex":[0.9981687,0.0001778111,0.000683526,0.0002167416,0.000417016,0.000336194],"domain_scores_gemma":[0.9988998,0.0001480306,0.000193373,0.0003066559,0.0003408672,0.0001113144],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001047162,0.0003538185,0.005779027,0.00005499047,0.00002930841,0.000003350228,0.00007862641,0.000002148792,0.9901332,0.00002611275,0.0004462127,0.002046028],"study_design_scores_gemma":[0.002071963,0.0001932395,0.001189891,0.00008429182,0.00002921349,0.000113024,0.0003092377,0.0000483774,0.9950381,0.00001641982,0.0007878544,0.000118373],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9957735,0.003046962,0.0004146415,0.0004152279,0.00005273323,0.000138456,0.00003239556,0.000001635082,0.0001244286],"genre_scores_gemma":[0.998447,0.0004263133,0.000444983,0.00005416621,0.0005776819,0.000008781523,0.00001870854,0.0000114644,0.00001087935],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004904898,"threshold_uncertainty_score":0.467233,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007303272560831952,"score_gpt":0.2740974292734295,"score_spread":0.2667941567125975,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}