{"id":"W2010712369","doi":"10.1016/s0958-1669(02)00016-2","title":"Ultrasensitive protein–DNA binding assays","year":2003,"lang":"en","type":"review","venue":"Current Opinion in Biotechnology","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":36,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Molecular beacon; DNA; Aptamer; Oligonucleotide; Capillary electrophoresis; Analyte; Affinity electrophoresis; DNA-binding protein; Chemistry; Electrophoretic mobility shift assay; Fluorescence anisotropy; Affinity chromatography; Molecular biology; Biochemistry; Biology; Chromatography; Enzyme; Transcription factor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002426293,0.003370504,0.002735231,0.002780898,0.0004822166,0.00145145,0.004927984,0.002111122,0.003803589],"category_scores_gemma":[0.001198683,0.001112295,0.0007943757,0.002025729,0.001669798,0.002289163,0.001211311,0.003940451,0.007815619],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001270804,"about_ca_system_score_gemma":0.0008339063,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007386774,"about_ca_topic_score_gemma":0.001176373,"domain_scores_codex":[0.9982529,0.0002867461,0.00008105457,0.000366298,0.0008411163,0.00017187],"domain_scores_gemma":[0.9989635,0.0005194045,0.00009714563,0.00007762754,0.0002834222,0.00005890382],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001658033,0.0002606507,0.0002980855,0.004708935,0.0000721199,0.0003328993,0.0001311407,0.000592625,0.2452949,0.01075914,0.02155126,0.7158325],"study_design_scores_gemma":[0.00004095042,0.0002161834,0.001071571,0.0004880317,0.000122979,0.002003844,0.00009922528,0.001480288,0.2343726,0.006302834,0.7536808,0.0001208032],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.004496435,0.7696909,0.1949711,0.001866613,0.003917033,0.0003523274,0.0004247263,0.001764038,0.02251687],"genre_scores_gemma":[0.02900381,0.7735507,0.1093297,0.004822624,0.002047153,0.0007119955,0.001590393,0.0001709602,0.07877266],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.004927984,"threshold_uncertainty_score":0.01283163,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05533163066857206,"score_gpt":0.3779651462059245,"score_spread":0.3226335155373524,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}