{"id":"W2010752031","doi":"10.1128/jb.05711-11","title":"Functional Characterization and Localization of a Bacillus subtilis Sortase and Its Substrate and Use of This Sortase System To Covalently Anchor a Heterologous Protein to the B. subtilis Cell Wall for Surface Display","year":2011,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Virginia Polytechnic Institute and State University","keywords":"Sortase; Bacillus subtilis; Sortase A; Biology; Cell wall; Biochemistry; Biophysics; Bacteria; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002538575,0.0003736054,0.0003114378,0.0001661332,0.000153064,0.0003789844,0.0002020335,0.0004261827,0.0006009572],"category_scores_gemma":[0.0002355163,0.0001746542,0.0003609935,0.000254634,0.0001817878,0.0002128875,0.0002055714,0.0007289303,0.0004746839],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002606967,"about_ca_system_score_gemma":0.0002733175,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009135042,"about_ca_topic_score_gemma":0.0007911609,"domain_scores_codex":[0.9998072,0.00003154872,0.00002697769,0.00002921538,0.00007884837,0.00002626568],"domain_scores_gemma":[0.9998119,0.00003908865,0.00003997136,0.00002722446,0.00003928747,0.00004250027],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001874378,0.000008828217,0.00005086076,0.00001629067,0.000001782239,0.00001256161,0.000007003363,0.0000216974,0.9993618,0.00003952889,0.000007011643,0.0004538863],"study_design_scores_gemma":[0.000003920049,0.00008173089,0.001235465,0.000002560252,0.000006875894,0.0001622149,0.00001722936,0.0004548452,0.9968136,0.00001688555,0.001200565,0.000004138416],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9756569,0.001004215,0.02093748,0.00019036,0.00002969192,0.00006291262,0.0009955413,0.0000845129,0.001038454],"genre_scores_gemma":[0.9647433,0.001069832,0.02705586,0.00006370994,0.000009261767,0.00006945372,0.003614225,0.00006975921,0.003304501],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009135042,"threshold_uncertainty_score":0.002010405,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01995584254032854,"score_gpt":0.2091074970867003,"score_spread":0.1891516545463718,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}