{"id":"W2010920240","doi":"10.1042/ba20070174","title":"Large‐scale plasmid DNA processing: evidence that cell harvesting and storage methods affect yield of supercoiled plasmid DNA","year":2008,"lang":"en","type":"article","venue":"Biotechnology and Applied Biochemistry","topic":"Membrane Separation Technologies","field":"Environmental Science","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"PricewaterhouseCoopers (Canada)","funders":"Engineering and Physical Sciences Research Council","keywords":"Plasmid; DNA; Plasmid preparation; Yield (engineering); Biology; DNA supercoil; Chemistry; Genetics; Materials science; DNA replication","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001064654,0.0006442696,0.0004227055,0.0003176057,0.0001936682,0.0007801125,0.0005109371,0.0007728615,0.001143187],"category_scores_gemma":[0.002740117,0.0002718087,0.0004564605,0.000475192,0.0003782971,0.0006854688,0.000488015,0.000635131,0.0006594225],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001967731,"about_ca_system_score_gemma":0.0002281729,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003492311,"about_ca_topic_score_gemma":0.0003862444,"domain_scores_codex":[0.9992024,0.000169706,0.00009816568,0.0001590436,0.0002950997,0.00007563161],"domain_scores_gemma":[0.9952354,0.002569948,0.001045756,0.0004454804,0.0005406484,0.0001628814],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001360763,0.00006903744,0.001230967,0.00007336015,0.00001812485,0.00003844821,0.00003521839,0.00008064339,0.9966245,0.00000456184,0.00001923477,0.001669949],"study_design_scores_gemma":[0.00000544536,0.0007009179,0.01663322,0.000007366629,0.00003298435,0.0001425822,0.0000371486,0.0003757741,0.9817774,0.00001561496,0.0002639087,0.000007618702],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9914919,0.00157828,0.005661374,0.0001105772,0.00002237486,0.00008403757,0.0004540001,0.00009835319,0.000499084],"genre_scores_gemma":[0.9905095,0.001095842,0.005700219,0.0001708795,0.00001743701,0.00009614391,0.001257849,0.0001158827,0.001036301],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001143187,"threshold_uncertainty_score":0.005630493,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02867934277626912,"score_gpt":0.2638034028480899,"score_spread":0.2351240600718208,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}