{"id":"W2011060772","doi":"10.1021/ac1003272","title":"Quantification of Viable but Nonculturable <i>Escherichia coli</i> O157:H7 by Targeting the <i>rpoS</i> mRNA","year":2010,"lang":"en","type":"letter","venue":"Analytical Chemistry","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"rpoS; Escherichia coli; Viable but nonculturable; Chemistry; Bacteria; Real-time polymerase chain reaction; Enterobacteriaceae; Messenger RNA; Gene; Microbiology; RNA; Biology; Molecular biology; Gene expression; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009518774,0.001128607,0.0007976764,0.000653524,0.0001966938,0.0009455047,0.0006020249,0.0007892655,0.0007800533],"category_scores_gemma":[0.0009953703,0.000396114,0.0003939519,0.0003256785,0.0005378864,0.0004556739,0.0004205303,0.0008815402,0.0006774072],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003444072,"about_ca_system_score_gemma":0.0003632672,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000356705,"about_ca_topic_score_gemma":0.0009187362,"domain_scores_codex":[0.9987175,0.0002138644,0.00009998724,0.0003198627,0.0005418045,0.0001069351],"domain_scores_gemma":[0.998897,0.0003669597,0.0002922032,0.00009338291,0.0002954439,0.00005508231],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001601742,0.00001854876,0.0004821329,0.00007252656,0.000003934108,0.00001304274,0.00002197226,0.00007294609,0.9972984,0.00004118342,0.00002691739,0.001932276],"study_design_scores_gemma":[0.000002136778,0.0001239849,0.002806301,0.00002547936,0.00001570151,0.00006975958,0.00004784356,0.001117205,0.9947134,0.0000553872,0.001013029,0.000009811158],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7483009,0.003877013,0.2390931,0.0002990109,0.0002205082,0.0003918257,0.002397029,0.001286145,0.004134524],"genre_scores_gemma":[0.7073731,0.0055966,0.2721888,0.0003811852,0.0001079535,0.0007848794,0.005471725,0.0004317283,0.007663969],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001128607,"threshold_uncertainty_score":0.005034029,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01126511776141687,"score_gpt":0.2547275214317207,"score_spread":0.2434624036703038,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}